AUTOMATIC SEGMENTATION OF EMBRYONIC HEART IN TIME-LAPSE FLUORESCENCE MICROSCOPY IMAGE SEQUENCES

被引：0

作者：

Kramer, P. ^{[1
]}

Boto, F. ^{[1
]}

Wald, D. ^{[1
]}

Bessy, F. ^{[1
]}

Paloc, C. ^{[1
]}

Callol, C. ^{[2
]}

Letamendia, A. ^{[2
]}

Ibarbia, I. ^{[2
]}

Holgado, O. ^{[2
]}

Virto, J. M. ^{[2
]}

机构：

[1] Vicomtech, Paseo Mikeletegi 57, San Sebastian 20009, Spain

[2] Biobide, San Sebastian 20009, Spain

来源：

BIOSIGNALS 2010: PROCEEDINGS OF THE THIRD INTERNATIONAL CONFERENCE ON BIO-INSPIRED SYSTEMS AND SIGNAL PROCESSING | 2010年

关键词：

Segmentation; Fluorescent microscopy images; Embryonic heart; ZEBRAFISH;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Embryos of animal models are becoming widely used to study cardiac development and genetics. However, the analysis of the embryonic heart is still mostly done manually. This is a very laborious and expensive task as each embryo has to be inspected visually by a biologist. We therefore propose to automatically segment the embryonic heart from high-speed fluorescence microscopy image sequences, allowing morphological and functional quantitative features of cardiac activity to be extracted. Several methods are presented and compared within a large range of images, varying in quality, acquisition parameters, and embryos position. Although manual control and visual assessment would still be necessary, the best of our methods has the potential to drastically reduce biologist workload by automating manual segmentation.

引用

页码：121 / 126

页数：6

共 47 条

[31] A geodesic deformable model for automatic segmentation of image sequences applied to radiation therapy
Bueno, G.
Deniz, O.
Salido, J.
Carrascosa, C.
Delgado, J. M.
INTERNATIONAL JOURNAL OF COMPUTER ASSISTED RADIOLOGY AND SURGERY, 2011, 6 (03) : 341 - 350
[32] Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
Zopf, Christopher J.
Maheshri, Narendra
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2013, (77):
[33] A computational 3D model for reconstruction of neural stem cells in bright-field time-lapse microscopy
Degerman, J.
Winterfors, E.
Faijerson, J.
Gustavsson, T.
COMPUTATIONAL IMAGING V, 2007, 6498
[34] Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy
Gfrerer, Lisa
Dougherty, Max
Liao, Eric C.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2013, (79): : e50525
[35] Stochastic Neural Networks for Automatic Cell Tracking in Microscopy Image Sequences of Bacterial Colonies
Sarmadi, Sorena
Winkle, James J.
Alnahhas, Razan N.
Bennett, Matthew R.
Josic, Kresimir
Mang, Andreas
Azencott, Robert
MATHEMATICAL AND COMPUTATIONAL APPLICATIONS, 2022, 27 (02)
[36] Isolated Random Forest Assisted Spatio-Temporal Ant Colony Evolutionary Algorithm for Cell Tracking in Time-Lapse Sequences
Xu, Benlian
Wu, Di
Shi, Jian
Cong, Jinliang
Lu, Mingli
Yang, Feng
Nener, Brett
IEEE JOURNAL OF BIOMEDICAL AND HEALTH INFORMATICS, 2024, 28 (07) : 4157 - 4169
[37] A graph-mining algorithm for automatic detection and counting of embryonic stem cells in fluorescence microscopy images
Faustino, Geisa M.
Gattass, Marcelo
de Lucena, Carlos J. P.
Campos, Priscila B.
Rehen, Stevens K.
INTEGRATED COMPUTER-AIDED ENGINEERING, 2011, 18 (01) : 91 - 106
[38] Fully Automatic Registration and Segmentation of First-Pass Myocardial Perfusion MR Image Sequences
Gupta, Vikas
Hendriks, Emile A.
Milles, Julien
van der Geest, Rob J.
Jerosch-Herold, Michael
Reiber, Johan H. C.
Lelieveldt, Boudewijn P. F.
ACADEMIC RADIOLOGY, 2010, 17 (11) : 1375 - 1385
[39] 3-D Active Meshes: Fast Discrete Deformable Models for Cell Tracking in 3-D Time-Lapse Microscopy
Dufour, Alexandre
Thibeaux, Roman
Labruyere, Elisabeth
Guillen, Nancy
Olivo-Marin, Jean-Christophe
IEEE TRANSACTIONS ON IMAGE PROCESSING, 2011, 20 (07) : 1925 - 1937
[40] Automated profiling of individual cell-cell interactions from high-throughput time-lapse imaging microscopy in nanowell grids (TIMING)
Merouane, Amine
Rey-Villamizar, Nicolas
Lu, Yanbin
Liadi, Ivan
Romain, Gabrielle
Lu, Jennifer
Singh, Harjeet
Cooper, Laurence J. N.
Varadarajan, Navin
Roysam, Badrinath
BIOINFORMATICS, 2015, 31 (19) : 3189 - 3197

← 1 2 3 4 5 →