Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals

被引：27

作者：

Wei, Ting ^{[1
,2
]}

Zhang, Chao ^{[1
,2
]}

Xu, Xin ^{[3
]}

Hanna, Michelle ^{[4
]}

Zhang, Xiaohua ^{[1
]}

Wang, Yan ^{[1
]}

Dai, Heping ^{[1
]}

Xiao, Wei ^{[3
,4
]}

机构：

[1] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China

[4] Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada

来源：

BIOSENSORS & BIOELECTRONICS | 2013年 / 44卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

Genotoxin; Reporter; Biosensor; Yeast; Sensitivity; PROMOTER-REPORTER CONSTRUCT; GREEN FLUORESCENT PROTEIN; INDUCED GENE-EXPRESSION; DNA-DAMAGE; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; TESTING SYSTEM; ENHANCES SENSITIVITY; CWP GENES; ASSAY;

D O I：

10.1016/j.bios.2013.01.029

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

It has been well established that essentially all microbial mutagens are rodent carcinogens, yet current mutagen detection systems are limited by their detection sensitivity. Here we report the construction of a pair of hypersensitive biosensors by optimizing both reporters and the host strain. The resulting RNR3-yEGFP and HUG1-yEGFP reporters and the septuple yeast mutant in combination with the automated protocol not only remarkably enhance the detection sensitivity, but also allow a high throughput screen of environmental genotoxins. This system is deemed much more sensitive than similar yeast and bacterium-based tests for all selected chemicals examined in this study. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：138 / 145

页数：8

共 39 条

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