Enzymatic transesterification of waste vegetable oil to produce biodiesel

被引:58
作者
Lopresto, C. G. [1 ]
Naccarato, S. [1 ,2 ]
Albo, L. [1 ]
De Paola, M. G. [1 ]
Chakraborty, S. [2 ]
Curcio, S. [2 ]
Calabro, V. [2 ]
机构
[1] Univ Calabria, Dept Mech Energy & Management Engn DIMEG, I-87036 Arcavacata Di Rende, CS, Italy
[2] Univ Calabria, Dept Informat Modeling Elect & Syst Engn DIMES, I-87036 Arcavacata Di Rende, CS, Italy
关键词
Biodiesel; Enzymatic transesterification; Immobilized lipase; Waste vegetable oil; FUEL PRODUCTION; LIPASE;
D O I
10.1016/j.ecoenv.2015.03.028
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
An experimental study on enzymatic transesterification was performed to produce biodiesel from waste vegetable oils. Lipase from Pseudomonas cepacia was covalently immobilized on a epoxy-acrylic resin support. The immobilized enzyme exhibited high catalytic specific surface and allowed an easy recovery, regeneration and reutilisation of biocatalyst. Waste vegetable oils - such as frying oils, considered not competitive with food applications and wastes to be treated - were used as a source of glycerides. Ethanol was used as a short chain alcohol and was added in three steps with the aim to reduce its inhibitory effect on lipase activity. The effect of biocatalyst/substrate feed mass ratios and the waste oil quality have been investigated in order to estimate the process performances. Biocatalyst recovery and reuse have been also studied with the aim to verify the stability of the biocatalyst for its application in industrial scale. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码:229 / 235
页数:7
相关论文
共 19 条
[1]   Proposed kinetic mechanism of the production of biodiesel from palm oil using lipase [J].
Al-Zuhair, Sulaiman ;
Ling, Fan Wei ;
Jun, Lim Song .
PROCESS BIOCHEMISTRY, 2007, 42 (06) :951-960
[2]   Repeated use of whole-cell biocatalysts immobilized within biomass support particles for biodiesel fuel production [J].
Ban, K ;
Hama, S ;
Nishizuka, K ;
Kaieda, M ;
Matsumoto, T ;
Kondo, A ;
Noda, H ;
Fukuda, H .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2002, 17 (3-5) :157-165
[3]   Whole cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles [J].
Ban, K ;
Kaieda, M ;
Matsumoto, T ;
Kondo, A ;
Fukuda, H .
BIOCHEMICAL ENGINEERING JOURNAL, 2001, 8 (01) :39-43
[4]   Kinetics of enzymatic trans-esterification of glycerides for biodiesel production [J].
Calabro, Vincenza ;
Ricca, Emanuele ;
De Paola, Maria Gabriela ;
Curcio, Stefano ;
Iorio, Gabriele .
BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2010, 33 (06) :701-710
[5]   Biodiesel from microalgae [J].
Chisti, Yusuf .
BIOTECHNOLOGY ADVANCES, 2007, 25 (03) :294-306
[6]   Factor analysis of transesterification reaction of waste oil for biodiesel production [J].
De Paola, M. G. ;
Ricca, E. ;
Calabro, V. ;
Curcio, S. ;
Iorio, G. .
BIORESOURCE TECHNOLOGY, 2009, 100 (21) :5126-5131
[7]   ESTER REACTIONS OF FATTY MATERIALS [J].
FORMO, MW .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1954, 31 (11) :548-559
[8]   TRANSESTERIFICATION KINETICS OF SOYBEAN OIL [J].
FREEDMAN, B ;
BUTTERFIELD, RO ;
PRYDE, EH .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1986, 63 (10) :1375-1380
[10]   Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent [J].
Kaieda, M ;
Samukawa, T ;
Matsumoto, T ;
Ban, K ;
Kondo, A ;
Shimada, Y ;
Noda, H ;
Nomoto, F ;
Ohtsuka, K ;
Izumoto, E ;
Fukuda, H .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 88 (06) :627-631