Generation of a Broadly Cross-Neutralizing Antibody Fragment against Several Mexican Scorpion Venoms

被引：20

作者：

Riano-Umbarila, Lidia ^{[1
]}

Gomez-Ramirez, Ilse V. ^{[2
]}

Ledezma-Candanoza, Luis M. ^{[2
]}

Olamendi-Portugal, Timoteo ^{[2
]}

Remi Rodriguez-Rodriguez, Everardo ^{[2
]}

Fernandez-Taboada, Guillermo ^{[2
]}

Possani, Lourival D. ^{[2
]}

Becerril, Baltazar ^{[2
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, CONACYT, Apartado Postal 510-3, Cuernavaca 62250, Morelos, Mexico

[2] UNAM, Inst Biotecnol, Dept Med Mol & Bioproc, Apartado Postal 510-3, Cuernavaca 62250, Morelos, Mexico

来源：

TOXINS | 2019年 / 11卷 / 01期

关键词：

cross-neutralization; directed evolution; phage display; polyvalent antivenom; scFv; scorpion; AMINO-ACID-SEQUENCE; 2; TOXINS; CENTRUROIDES; ACTIVATION; PEPTIDES; SUFFUSUS; CHANNEL; PROTEIN;

D O I：

10.3390/toxins11010032

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The recombinant antibody fragments generated against the toxic components of scorpion venoms are considered a promising alternative for obtaining new antivenoms for therapy. Using directed evolution and site-directed mutagenesis, it was possible to generate a human single-chain antibody fragment with a broad cross-reactivity that retained recognition for its original antigen. This variant is the first antibody fragment that neutralizes the effect of an estimated 13 neurotoxins present in the venom of nine species of Mexican scorpions. This single antibody fragment showed the properties of a polyvalent antivenom. These results represent a significant advance in the development of new antivenoms against scorpion stings, since the number of components would be minimized due to their broad cross-neutralization capacity, while at the same time bypassing animal immunization.

引用

页数：17

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