Ethanol weakens cytochalasin B binding to the GLUT1 glucose transporter and drug partitioning into lipid bilayers

被引：4

作者：

Hägglund, CL ^{[1
]}

Gottschalk, I ^{[1
]}

Lundahl, P ^{[1
]}

机构：

[1] Uppsala Univ, Biomed Ctr, Dept Biochem, SE-75123 Uppsala, Sweden

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1031卷 / 1-2期

关键词：

immobilized biomembrane affinity chromatography; cytochalasin B; ethanol; glucose; GLUT1;

D O I：

10.1016/j.chroma.2003.10.132

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ethanol weakens the specific interaction between the human red blood cell (RBC) glucose transporter GLUT1 and the inhibitor cytochalasin B (CB). The chromatographic retention volume of cytochalasin B on stationary phases consisting of GLUT1-containing membranes decreased with increasing ethanol concentration in the eluent. The apparent K-d values for the ethanol-GLUT1 interaction were 0.37, 0.45 and 0.64 M for red blood cells, red blood cell membrane vesicles and proteoliposomes, respectively, all much higher than the K-d values for D-glucose or cytochalasin B interaction with GLUT1. Ethanol also decreased the partitioning of cytochalasin B and drugs into phospholipid bilayers. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：113 / 116

页数：4

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