Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

被引：24

作者：

Li, Pei-jun ^{[1
]}

Xia, Jin-lan ^{[1
]}

Shan, Yang ^{[2
]}

Nie, Zhen-yuan ^{[1
]}

机构：

[1] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China

[2] Hunan Acad Agr Sci, Changsha 410125, Hunan, Peoples R China

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2015年 / 38卷 / 10期

基金：

国家高技术研究发展计划(863计划);

关键词：

Multi-enzyme complexes; Solid-state fermentation; Submerged fermentation; Ultrasound; Zymography; SOLID-STATE FERMENTATION; CELLULASE PRODUCTION; XYLANASE PRODUCTION; SUBSTRATE; OPTIMIZATION; PECTINASE; CULTURE; SSF;

D O I：

10.1007/s00449-015-1442-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Submerged fermentation (SmF) and solid-state fermentation (SSF) of Aspergillus japonicus PJ01 for multi-enzyme complexes (MEC) production were comparatively studied. The results showed that orange peel and wheat bran were the best substrates for MEC production in SmF and SSF, respectively. After 72 h of cultivation under SmF, the maximal pectinase, CMCase, and xylanase activities reached 2610, 85, and 335 U/gds (units/gram dry substrate), respectively; while after 72 h of cultivation under SSF, these three enzymes' activities reached 966, 58, and 1004 U/gds, respectively. Effects of ultrasound on extraction of crude enzymes from SSF medium were determined, the maximal activities of pectinase, CMCase, and xylanase increased to 1.20, 1.48, and 1.30-fold, respectively. Apparent different mycelia growths of SSF and SmF were observed by scanning electron microscopy; and different isoforms of the crude enzyme extracts from SSF and SmF were presented by zymogram analysis.

引用

页码：2013 / 2022

页数：10

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