Isolation of lipids from biological samples

被引：39

作者：

Furse, Samuel ^{[1
]}

Egmond, Maarten R. ^{[1
]}

Killian, J. Antoinette ^{[1
]}

机构：

[1] Univ Utrecht, Membrane Biochem & Biophys, NL-3584 CH Utrecht, Netherlands

来源：

MOLECULAR MEMBRANE BIOLOGY | 2015年 / 32卷 / 03期

关键词：

Lipases; lipid; lipidomics; lipid chemistry; lipid extraction; lipid isolation; molecular profiling; phosphatases; FATTY-ACID-COMPOSITION; TRIGLYCERIDE COMPOSITION; STEREOSPECIFIC ANALYSIS; MASS-SPECTROMETRY; ESCHERICHIA-COLI; SACCHAROMYCES-CEREVISIAE; LIPIDOMICS REVEALS; PLASMA-MEMBRANE; PROTEIN-KINASE; MILK-FAT;

D O I：

10.3109/09687688.2015.1050468

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isolation of the lipid fraction from biological samples has been a crucial part of countless studies over the last century. This considerable research interest has led to the development of a number of methods for isolating a range of molecular species that fall under the umbrella term "lipid''. Such methods vary in popularity, complexity, specificity and even toxicity. In this review, we explore examples of published methods (1952-2014) for isolating lipids from biological samples and attempt to assess the limits of techniques both from a chemical and biological perspective. We also suggest how a suitable method might be chosen for a novel application.

引用

页码：55 / 64

页数：10

共 116 条

[1] Lipase-catalyzed interesterification of soybean oil with an ω-3 polyunsaturated fatty acid concentrate prepared from sardine oil [J].

Akimoto, M ;

Izawa, M ;

Hoshino, K ;

Abe, KI ;

Takahashi, H .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2003, 104 (02) :105-118

[2] TURNOVER OF INOSITOL AND PHOSPHORUS CONTAINING LIPIDS IN SACCHAROMYCES-CEREVISIAE - EXTRACELLULAR ACCUMULATION OF GLYCEROPHOSPHORYLINOSITOL DERIVED FROM PHOSPHATIDYLINOSITOL [J].

ANGUS, WW ;

LESTER, RL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1972, 151 (02) :483-&

[3] Caveolae regulate the nanoscale organization of the plasma membrane to remotely control Ras signaling [J].

Ariotti, Nicholas ;

Fernandez-Rojo, Manuel A. ;

Zhou, Yong ;

Hill, Michelle M. ;

Rodkey, Travis L. ;

Inder, Kerry L. ;

Tanner, Lukas B. ;

Wenk, Markus R. ;

Hancock, John F. ;

Parton, Robert G. .

JOURNAL OF CELL BIOLOGY, 2014, 204 (05) :777-792

[4] The main triglyceride-lipase from the insect fat body is an active phospholipase A1:: identification and characterization [J].

Arrese, Estela L. ;

Patel, Rajesh T. ;

Soulages, Jose L. .

JOURNAL OF LIPID RESEARCH, 2006, 47 (12) :2656-2667

[5] Dividing Cells Regulate Their Lipid Composition and Localization [J].

Atilla-Gokcumen, G. Ekin ;

Muro, Eleonora ;

Relat-Goberna, Josep ;

Sasse, Sofia ;

Bedigian, Anne ;

Coughlin, Margaret L. ;

Garcia-Manyes, Sergi ;

Eggert, Ulrike S. .

CELL, 2014, 156 (03) :428-439

[6]

AUGERT G, 1989, J BIOL CHEM, V264, P2574

[7] Direct quantitative analysis of lysophosphatidic acid molecular species by stable isotope dilution electrospray ionization liquid chromatography-mass spectrometry [J].

Baker, DL ;

Desiderio, DM ;

Miller, DD ;

Tolley, B ;

Tigyi, GJ .

ANALYTICAL BIOCHEMISTRY, 2001, 292 (02) :287-295

[8] Lipidomics reveals that adiposomes store ether lipids and mediate phospholipid traffic [J].

Bartz, Rene ;

Li, Wen-Hong ;

Venables, Barney ;

Zehmer, John K. ;

Roth, Mary R. ;

Welti, Ruth ;

Anderson, Richard G. W. ;

Liu, Pingsheng ;

Chapman, Kent D. .

JOURNAL OF LIPID RESEARCH, 2007, 48 (04) :837-847

[9] EFFECTS OF VARIOUS DIETARY FATS ON CARDIOLIPIN ACYL COMPOSITION DURING ONTOGENY OF MICE [J].

BERGER, A ;

GERSHWIN, ME ;

GERMAN, JB .

LIPIDS, 1992, 27 (08) :605-612

[10] INOSITOL TRISPHOSPHATE, A NOVEL 2ND MESSENGER IN CELLULAR SIGNAL TRANSDUCTION [J].

BERRIDGE, MJ ;

IRVINE, RF .

NATURE, 1984, 312 (5992) :315-321

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