A novel monoclonal antibodies-based sandwich ELISA for detection of serotype 4 fowl adenovirus

被引：24

作者：

Shao, Hongxia ^{[1
,2
,3
]}

Wang, Ping ^{[1
,2
,3
]}

Wang, Weikang ^{[1
,2
,3
]}

Zhang, Jianjun ^{[4
]}

Li, Tuofan ^{[1
,2
,3
]}

Liang, Guangchen ^{[1
,2
,3
]}

Gao, Wei ^{[1
,2
,3
]}

Qin, Aijian ^{[1
,2
,3
]}

Ye, Jianqiang ^{[1
,2
,3
]}

机构：

[1] Yangzhou Univ, Key Lab Jiangsu Prevent Vet Med, Key Lab Avian Prevent Med, Minist Educ,Coll Vet Med, 12 East Wenhui Rd, Yangzhou 225009, Jiangsu, Peoples R China

[2] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

[3] Yangzhou Univ, Joint Int Res Lab Agr & Agri Prod Safety, Minist Educ Peoples Republ China, Yangzhou 225009, Jiangsu, Peoples R China

[4] Sinopharm Yangzhou VAC Biol Engn Co Ltd, Yangzhou, Jiangsu, Peoples R China

来源：

AVIAN PATHOLOGY | 2019年 / 48卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Serotype 4 fowl adenovirus; monoclonal antibody; sandwich ELISA; RECOMBINANT FIBER-2 PROTEIN; HYDROPERICARDIUM SYNDROME; CHICKENS; HEPATITIS;

D O I：

10.1080/03079457.2019.1566595

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

As a major causative agent for hepatitis-hydropericardium syndrome (HPS) in chickens, serotype 4 fowl adenovirus (FAdV-4) has caused huge economic losses in the poultry industry globally. However, there is no commercial diagnostic test for FAdV-4 antigens. To generate a rapid approach for specific detection of FAdV-4, a monoclonal antibodies (mAbs)-based sandwich ELISA was developed. In this ELISA, a purified mAb 4A3 and a HRP-labelled mAb 3C2 specific to the fiber-2 of FAdV-4 were used as the capture antibody and detection antibody respectively. Specificity assay revealed the ELISA only reacted with FAdV-4, not with other avian viruses tested. Sensitivity assay showed the limit of detection of the ELISA was 1000 TCID50/ml and 12.5 ng/ml for the FAdV-4 and the purified GST-Fiber2 protein respectively. Moreover, the ELISA could be efficiently applied in detecting the FAdV-4 in tissue samples from a clinically-diseased chicken flock. All these data demonstrated that the ELISA developed here provides a promising tool for rapid and efficient diagnosis of clinical infection with FAdV-4.

引用

页码：204 / 208

页数：5