Replication-coupled passive DNA demethylation for the erasure of genome imprints in mice

被引：230

作者：

Kagiwada, Saya ^{[1
]}

Kurimoto, Kazuki ^{[1
,2
]}

Hirota, Takayuki ^{[1
,2
]}

Yamaji, Masashi ^{[1
,2
]}

Saitou, Mitinori ^{[1
,2
,3
,4
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Anat & Cell Biol, Kyoto 6068501, Japan

[2] JST, ERATO, Kyoto, Japan

[3] Kyoto Univ, Ctr iPS Cell Res & Applicat, Kyoto 6068501, Japan

[4] Kyoto Univ, Inst Integrated Cell Mat Sci, Kyoto 6068501, Japan

来源：

EMBO JOURNAL | 2013年 / 32卷 / 03期

关键词：

cell cycle; chromatin dynamics; genome imprints; primordial germ cells; replication-coupled DNA; demethylation; PRIMORDIAL GERM-CELLS; EPIGENETIC INHERITANCE; CDNA AMPLIFICATION; DYNAMICS; SPECIFICATION; METHYLATION; 5-METHYLCYTOSINE; EXPRESSION; GENE; CHROMATIN;

D O I：

10.1038/emboj.2012.331

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genome-wide DNA demethylation, including the erasure of genome imprints, in primordial germ cells (PGCs) is a critical first step to creating a totipotent epigenome in the germ line. We show here that, contrary to the prevailing model emphasizing active DNA demethylation, imprint erasure in mouse PGCs occurs in a manner largely consistent with replication-coupled passive DNA demethylation: PGCs erase imprints during their rapid cycling with little de novo or maintenance DNA methylation potential and no apparent major chromatin alterations. Our findings necessitate the re-evaluation of and provide novel insights into the mechanism of genome-wide DNA demethylation in PGCs.

引用

页码：340 / 353

页数：14

共 53 条

[1] Polycomb group protein-associated chromatin is reproduced in post-mitotic G1 phase and is required for S phase progression [J].

Aoto, Takahiro ;

Saitoh, Noriko ;

Sakamoto, Yasuo ;

Watanabe, Sugiko ;

Nakao, Mitsuyoshi .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (27) :18905-18915

[2] UHRF1 plays a role in maintaining DNA methylation in mammalian cells [J].

Bostick, Magnolia ;

Kim, Jong Kyong ;

Esteve, Pierre-Olivier ;

Clark, Amander ;

Pradhan, Sriharsa ;

Jacobsen, Steven E. .

SCIENCE, 2007, 317 (5845) :1760-1764

[3] The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development [J].

Chang, DH ;

Calame, KL .

MECHANISMS OF DEVELOPMENT, 2002, 117 (1-2) :305-309

[4] Tet1 Is Dispensable for Maintaining Pluripotency and Its Loss Is Compatible with Embryonic and Postnatal Development [J].

Dawlaty, Meelad M. ;

Ganz, Kibibi ;

Powell, Benjamin E. ;

Hu, Yueh-Chiang ;

Markoulaki, Styliani ;

Cheng, Albert W. ;

Gao, Qing ;

Kim, Jongpil ;

Choi, Sang-Woon ;

Page, David C. ;

Jaenisch, Rudolf .

CELL STEM CELL, 2011, 9 (02) :166-175

[5] Epigenetic Reprogramming in Plant and Animal Development [J].

Feng, Suhua ;

Jacobsen, Steven E. ;

Reik, Wolf .

SCIENCE, 2010, 330 (6004) :622-627

[6] A new class of uracil-DNA glycosylases related to human thymine-DNA glycosylase [J].

Gallinari, P ;

Jiricny, J .

NATURE, 1996, 383 (6602) :735-738

[7] Dnmt1 expression in pre- and postimplantation embryogenesis and the maintenance of IAP silencing [J].

Gaudet, F ;

Rideout, WM ;

Meissner, A ;

Dausman, J ;

Leonhardt, H ;

Jaenisch, R .

MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (04) :1640-1648

[8] Global profiling of DNA methylation erasure in mouse primordial germ cells [J].

Guibert, Sylvain ;

Forne, Thierry ;

Weber, Michael .

GENOME RESEARCH, 2012, 22 (04) :633-641

[9] Hydroxylation of 5-Methylcytosine by TET1 Promotes Active DNA Demethylation in the Adult Brain [J].

Guo, Junjie U. ;

Su, Yijing ;

Zhong, Chun ;

Ming, Guo-li ;

Song, Hongjun .

CELL, 2011, 145 (03) :423-434

[10] Epigenetic reprogramming in mouse primordial germ cells [J].

Hajkova, P ;

Erhardt, S ;

Lane, N ;

Haaf, T ;

El-Maarri, O ;

Reik, W ;

Walter, J ;

Surani, MA .

MECHANISMS OF DEVELOPMENT, 2002, 117 (1-2) :15-23

← 1 2 3 4 5 6 →