Highly selective DNA biosensor based on the long-range electron transfer of indigo carmine through DNA duplex

被引：5
作者：
Wang, Qingxiang [1 ,2 ]
Ding, Yingtao [2 ]
Wang, Liheng [2 ]
Gao, Cai [2 ]
Gao, Feng [2 ]
Gao, Fei [2 ]
机构：
[1] Zhangzhou Normal Univ, Fujian Prov Univ Key Lab Analyt Sci, Zhangzhou 363000, Peoples R China
[2] Zhangzhou Normal Univ, Dept Chem & Environm Sci, Zhangzhou 363000, Peoples R China
来源：
MICROCHIMICA ACTA | 2012年 / 179卷 / 3-4期
基金：
中国国家自然科学基金;
关键词：
DNA biosensor; Indigo carmine; Long-range electron transfer; One-base mismatch; DOUBLE-STRANDED DNA; ELECTROCHEMICAL DETECTION; HYBRIDIZATION; COMPLEX; RECOGNITION; SENSOR; INDICATOR; BINDING; GENE; BLUE;
D O I：
10.1007/s00604-012-0892-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have developed a highly selective DNA biosensor. It was based on the long-range electron transfer (LRET) from the electroactive dye indigo carmine (IC) through the DNA duplex on a glassy carbon electrode. Voltammetric experiments showed that IC interacts with dsDNA through a typical intercalative mode with a relatively strong affinity of 2.3(+/- 0.6) x 10(6) M-1. If incubated with DNA in IC solution, no response was observed with the ssDNA-modified probe electrode. However, a pair of well-defined redox peak was observed with a DNA-hybridized electrode, proving the presence of LRET on the biosensor. The biosensor also can differentiate complementary sequences, non-complementary sequences, and even the mutated sequences with single-base mismatches at different sites.
引用
收藏
页码：273 / 281
页数：9
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