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Rapid and Semiautomated Method for the Analysis of Veterinary Drug Residues in Honey Based on Turbulent-Flow Liquid Chromatography Coupled to Ultrahigh-Performance Liquid Chromatography-Orbitrap Mass Spectrometry (TFC-UHPLC-Orbitrap-MS)
被引:39
作者:
Aguilera-Luiz, Maria M.
[1
]
Romero-Gonzalez, Roberto
[1
]
Plaza-Bolanos, Patricia
[1
,2
]
Martinez Vidal, Jose L.
[1
]
Garrido Frenich, Antonia
[1
]
机构:
[1] Univ Almeria, Dept Chem & Phys, Analyt Chem Area, Res Ctr Agr & Food Biotechnol BITAL,CeiA3, E-04120 Almeria, Spain
[2] Univ Granada, Dept Analyt Chem, E-18071 Granada, Spain
关键词:
turbulent flow chromatography (TFC);
ultrahigh-performance liquid chromatography (UHPLC);
Orbitrap high-resolution mass spectrometry (HRMS);
multiclass analysis;
veterinary drugs;
honey;
SOLID-PHASE EXTRACTION;
MACROLIDE ANTIBIOTIC-RESIDUES;
TETRACYCLINE RESIDUES;
FLUORESCENCE DETECTION;
LC-MS/MS;
MILK;
ULTRAVIOLET;
VALIDATION;
GROWTH;
EGGS;
D O I:
10.1021/jf3048498
中图分类号:
S [农业科学];
学科分类号:
09 ;
摘要:
A simple and rapid method is described for the determination of veterinary drug (VD) residues in honey samples using turbulent flow chromatography coupled to ultrahigh-performance liquid chromatography-Orbitrap mass spectrometry (TFC-UHPLC-Orbitrap-MS). Honey samples were diluted with an aqueous solution of Na(2)EDTA (0.1 M). Then, they were injected into the chromatographic system including a TFC column. Afterward, the analytes were transferred to an UHPLC analytical column, where they were determined by UHPLC-Orbitrap-MS. Mean recoveries were obtained at three concentration levels (5, 10, and 50 mu g/kg), ranging from 68 to 121% for most compounds. Repeatability (intraday precision) and interday precision (expressed as relative standard deviation, RSD) were <2596 for most compounds. Limits of quantification (LOQs) ranged from 5 to 50 mu g/kg and limits of identification (LOIs) from 0.1 to 50 mu g/kg. The developed method was applied in honey samples, and it was fast and nonlaborious.
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页码:829 / 839
页数:11
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