TP53-induced glycolysis and apoptosis regulator is indispensable for mitochondria quality control and degradation following damage

被引：3

作者：

Feng, Jing ^{[1
]}

Luo, Li ^{[2
]}

Liu, Yong ^{[1
]}

Fu, Shaozhi ^{[1
]}

Chen, Jie ^{[3
]}

Duan, Xiaoxia ^{[4
]}

Xiang, Li ^{[1
]}

Zhang, Yanling ^{[1
]}

Wu, Jinbo ^{[1
]}

Fan, Juan ^{[1
]}

Wen, Qinglian ^{[1
]}

Zhang, Ye ^{[5
]}

Yang, Jingpin ^{[1
]}

Peng, Jinxia ^{[1
]}

Zhao, Ming ^{[1
]}

Yang, Linglin ^{[1
]}

机构：

[1] Southwest Med Univ, Affiliated Hosp, Dept Oncol, 25 Taiping St, Luzhou 646000, Sichuan, Peoples R China

[2] Southwest Med Univ, Affiliated Hosp, Dept Lab Med, Luzhou 646000, Sichuan, Peoples R China

[3] Southwest Med Univ, Affiliated Hosp, Dept Rheumatol & Immunol, Luzhou 646000, Sichuan, Peoples R China

[4] Southwest Med Univ, Affiliated Hosp, Dept Anesthesiol, Luzhou 646000, Sichuan, Peoples R China

[5] Southwest Med Univ, Affiliated Hosp, Dept Neurosurg, Luzhou 646000, Sichuan, Peoples R China

来源：

ONCOLOGY LETTERS | 2018年 / 15卷 / 01期

关键词：

TP53 induced glycolysis regulatory phosphatase; mitochondrial membrane potential; mitochondrial degradation; mitochondrial integrity; AUTOPHAGY; TIGAR; MITOPHAGY; STRESS; PARKIN;

D O I：

10.3892/ol.2017.7303

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Mitochondria have been described as 'the powerhouse of the cell' as the organelle generates the majority of adenosine triphosphate (ATP) in cells to support life. Mitochondria can be damaged due to stress, for example by reactive oxygen species (ROS). TP53-induced glycolysis and apoptosis regulator (TIGAR) serves a role in suppressing ROS damage and may protect mitochondria integrity. In the present study, the localization of TIGAR on mitochondria in 5-8F cells was demonstrated. Furthermore, it was indicated that the knockdown of TIGAR using lentivirus-short hairpin RNA induces the loss of mitochondrial membrane potential and cytochrome c leakage. However, these damaged mitochondria were not degraded in cells, but exhibited an abnormal appearance as indicated by mitochondrial swelling, crista collapse and vacuolization, with physiological dysfunction marked by reduced ATP production. Therefore, TIGAR maybe an indispensable protein for mitochondrial protection and degradation following cellular damage.

引用

页码：155 / 160

页数：6