Revealing the specific interactions between G-quadruplexes and ligands by surface-enhanced Raman spectroscopy

被引：3

作者：

Bao, Ying ^{[1
]}

Zhang, Xiaonong ^{[2
]}

Xiang, Xiaoxuan ^{[1
]}

Zhang, Yujing ^{[1
]}

Guo, Xinhua ^{[1
,3
]}

机构：

[1] Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, 2699 Qianjin St, Changchun 130012, Peoples R China

[2] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Jilin Biomed Polymers Engn Lab, Changchun 130022, Peoples R China

[3] Jilin Univ, Coll Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130012, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2022年 / 222卷

基金：

中国国家自然科学基金;

关键词：

SERS; G-quadruplex; Ligands; Interaction; TELOMERIC G-QUADRUPLEX; CONFORMATIONAL-CHANGES; STRUCTURE POLYMORPHISM; MASS-SPECTROMETRY; POLARIZED RAMAN; NUCLEIC-ACIDS;

D O I：

10.1016/j.ijbiomac.2022.10.070

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

G-quadruplexes (G4s) regulate a variety of physiological functions related to diseases and life elongation. Therefore, G4 binding ligands, such as potential drugs in gene therapy or molecular probes for biosensing and bioimaging, are receiving extensive attention. However, identifying the binding modes and interaction details between G4s and their ligands is very challenging. Recently, we demonstrated that surface-enhanced Raman scattering (SERS) could quickly provide structural details of G4s. Herein, three G4 binding ligands that interact with the separated G4 in different ways are selected as models to evaluate the feasibility of SERS analysis in studying G4-ligand interactions. As a result, adequate SERS information indicating the specific interactions between the G4s and the ligand is obtained via using Ag IANPs as substrates. The results demonstrate that SERS is a powerful tool for revealing comprehensive and specific ligand-DNA interactions with advantages such as speed, simplicity, trace sample amount requirement, and compatibility with aqueous samples.

引用

页码：2948 / 2956

页数：9

共 48 条

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