Involvement of ubiquitin proteasome system in protective mechanisms of Puerarin to MPP+-elicited apoptosis

被引：77

作者：

Cheng, Yue-Fa ^{[1
]}

Zhu, Guo-Qi ^{[2
]}

Wang, Mei ^{[2
]}

Cheng, Hui ^{[2
]}

Zhou, An ^{[2
]}

Wang, Ning ^{[2
]}

Fang, Nianbai ^{[3
]}

Wang, Xun-Cui ^{[2
]}

Xiao, Xiao-Qiu ^{[4
]}

Chen, Zhi-Wu ^{[1
]}

Li, Qing-Lin ^{[2
]}

机构：

[1] Anhui Med Univ, Dept Pharmacol, Hefei 230032, Peoples R China

[2] Anhui Univ Tradit Chinese Med, Anhui Key Lab Modernized Chinese Mat Med, Hefei 230038, Peoples R China

[3] Hubei Univ Tradit Chinese Med, Minist Educ, Key Lab Tradit Chinese Med Resource & Compound Pr, Wuhan 430065, Peoples R China

[4] Singapore Bioimaging Consortium, Lab Metab Med, Singapore 138667, Singapore

来源：

NEUROSCIENCE RESEARCH | 2009年 / 63卷 / 01期

关键词：

Parkinson's disease; Ubiquitin proteasome system; Apoptosis; Puerarin; ALPHA-SYNUCLEIN; SH-SY5Y CELLS; PROTEIN; AUTOPHAGY; DISEASE; DEATH; RAT;

D O I：

10.1016/j.neures.2008.10.009

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

It has been well documented that dysfunction of ubiquitin proteasome system (UPS) in the neuron exacerbated the Parkinson's disease (PD). However, whether or not UPS is involved in the protective effect of Puerarin on 1-Methyl-4-Phenyl-1, 2, 3, 6-Tetrahydropyridine (MPP+)-elicited cell death is yet to be elucidated. In this study, treatment of SH-SY5Y cells with 1 mM MPP+-elicited a characteristic apoptotic cell death and pretreatment with Puerarin protected cells against MPP+-induced apoptosis as evidenced by promoting cell viability, improving morphological changes and reducing apoptotic rate. To further explore the potential protective mechanism of Puerarin in MPP+-induced SH-SY5Y cell death, UPS activity, mitochondria-dependent apoptosis and caspase-3 activity were measured. Puerarin pretreatment attenuated MPP+-induced dysfunction of protease activity, thereby reducing accumulation of ubiquitin-conjugated proteins. Meanwhile, caspase-3 activity was remarkably attenuated by Puerarin. In addition, the ratio of bcl-2/bax was increased by Puerarin in comparison with MPP+-treated group. Taken together, these results suggest that Puerarin Could protect MPP+-induced SH-SY5Y cells from apoptosis by regulating the function of UPS. (C) 2008 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

引用

页码：52 / 58

页数：7

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