A simple artificial urine for the growth of urinary pathogens

被引：420

作者：

Brooks, T ^{[1
]}

Keevil, CW ^{[1
]}

机构：

[1] PUBL HLTH LAB SERV,CTR APPL MICROBIOL & RES,SALISBURY SP4 0JG,WILTS,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1997年 / 24卷 / 03期

关键词：

D O I：

10.1046/j.1472-765X.1997.00378.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10(8) cfu ml(-1), as found in normal urine. Reproducible, steady-state growth also occurred over many generations in continuous culture. AUM was capable of forming crystals and encrustations resembling those found in natural urinary tract infections. The medium is a suitable replacement for normal urine for use in a wide range of experiments modelling the growth and attachment of urinary pathogens in the clinical environment.

引用

页码：203 / 206

页数：4

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