Expression, purification, crystallization and preliminary X-ray diffraction of a novel Nitrosomonas europaea cytochrome, cytochrome P460

被引：9

作者：

Elmore, BO ^{[1
]}

Pearson, AR ^{[1
]}

Wilmot, CM ^{[1
]}

Hooper, AB ^{[1
]}

机构：

[1] Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2006年 / 62卷

关键词：

D O I：

10.1107/S1744309106008785

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytochrome P460 from Nitrosomonas europaea, a novel mono-heme protein containing an unusual cross-link between a conserved lysine and the porphyrin ring, has been recombinantly expressed and purified from Escherichia coli. The protein crystallizes readily and diffraction to 1.7 angstrom has been obtained in-house. The crystals belong to the trigonal space group P3(1/2)21, with unit-cell parameters a = b = 53.3, c = 127.1 angstrom, and contain one monomer in the asymmetric unit.

引用

页码：395 / 398

页数：4

共 37 条

[1] A COMPARISON OF NO AND N2O PRODUCTION BY THE AUTOTROPHIC NITRIFIER NITROSOMONAS-EUROPAEA AND THE HETEROTROPHIC NITRIFIER ALCALIGENES-FAECALIS
ANDERSON, IC
POTH, M
HOMSTEAD, J
BURDIGE, D
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (11) : 3525 - 3533
[2] P460 OF HYDROXYLAMINE OXIDOREDUCTASE OF NITROSOMONAS-EUROPAEA - SORET RESONANCE RAMAN EVIDENCE FOR A NOVEL HEME-LIKE STRUCTURE
ANDERSSON, KK
BABCOCK, GT
HOOPER, AB
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 174 (01) : 358 - 363
[3] ANDERSSON KK, 1984, J BIOL CHEM, V259, P6833
[4] Consideration of a phlorin structure for haem P-460 of hydroxylamine oxidoreductase and its implications regarding reaction mechanism
Arciero, DM
Hooper, AB
[J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1998, 26 (03) : 385 - 389
[5] ARCIERO DM, 1993, J BIOL CHEM, V268, P14645
[6] EVIDENCE FOR THE STRUCTURE OF THE ACTIVE-SITE HEME-P460 IN HYDROXYLAMINE OXIDOREDUCTASE OF NITROSOMONAS
ARCIERO, DM
HOOPER, AB
CAI, M
TIMKOVICH, R
[J]. BIOCHEMISTRY, 1993, 32 (36) : 9370 - 9378
[7] Overproduction of the Bradyrhizobium japonicum c-type cytochrome subunits of the cbb3 oxidase in Escherichia coli
Arslan, E
Schulz, H
Zufferey, R
Künzler, P
Thöny-Meyer, L
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 251 (03) : 744 - 747
[8] Ausubel F.M., 1992, SHORT PROTOCOLS MOL, V2nd
[9] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY
BAILEY, S
[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 : 760 - 763
[10] THE PRIMARY STRUCTURE OF CYTOCHROME P460 OF NITROSOMONAS-EUROPAEA - PRESENCE OF A C-HEME BINDING MOTIF
BERGMANN, DJ
HOOPER, AB
[J]. FEBS LETTERS, 1994, 353 (03) : 324 - 326

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