Structural and biochemical alterations during the healing process of tendons treated with Aloe vera

Aims: The tendon is composed of highly organized collagen fibers that form a complex supramolecular structure. After lesions, the organization and composition of the tendon are not completely restored. Our purpose was to evaluate if the application of Aloe vera improves tendon healing, considering the effectiveness in the stimulus of collagen synthesis. Main methods: The calcaneal tendon of male Wistar rats was partially transected with subsequent topical application of A. vera ointment at the injury. The animals were separated into groups with tendons treated with the A. vera extract for 7 days and excised on the 7th, 14th and 21st days after surgery: control rats received only ointment base without plant extract. Key findings: Morphological analysis using polarization microscopy showed that the entire tendon undergoes a remodeling process, with disorganized collagen fibers by days 7 and 14 in plant treated and non-treated groups and with a higher birefringence in tendons of the plant-treated group on the 21st day. A higher concentration of hydroxyproline was found in plant-treated tendons on days 7 and 14 compared with their controls. Western blots showed lower amounts of type I collagen in the plant-treated group on day 14 compared with the control. MMP-9 diminished 14 days after lesion and the active isoform of MMP-2 increased on day 21 in plant-treated groups. Significance: The present study indicates a beneficial effect of A. vera in the tissue reorganization in the transected region of the tendon 21 days after injury and is supported by an increase of active MMP-2. (C) 2012 Elsevier Inc. All rights reserved.