Construction of ratiometric fluorescent sensors by ribonucleopeptides

被引：7

作者：

Annoni, Chiara ^{[1
,3
]}

Nakata, Eiji ^{[1
,2
]}

Tamura, Tomoki ^{[1
]}

Liew, Fong Fong ^{[1
]}

Nakano, Shun ^{[1
]}

Gelmi, Maria Luisa ^{[3
]}

Morii, Takashi ^{[1
,2
]}

机构：

[1] Kyoto Univ, Grad Sch Energy Sci, Uji, Kyoto 6110011, Japan

[2] Kyoto Univ, Inst Adv Energy, Uji, Kyoto 6110011, Japan

[3] Univ Milan, Sez Chim Gen Organ A Marchesini, Dipartimento Sci Farmaceut Pietro Pratesi, I-20133 Milan, Italy

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2012年 / 10卷 / 44期

关键词：

RNA APTAMER; TETRACYCLINE; CONVERSION; BIOSENSORS; COMPLEXES; PROBE;

D O I：

10.1039/c2ob26722e

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Ratiometric fluorescent sensors were constructed from RNA aptamers by generating modular ribonucleopeptide complexes. Fluorescent ribonucleopeptides containing fluorophore seminaphthorhodafluor tethered to their peptide subunit revealed a dual emission property, which permitted a ratiometric fluorescent measurement of a substrate-binding event. The strategy successfully afforded ratiometric fluorescent sensors for biologically active small ligands, tetracycline, dopamine and streptomycin.

引用

页码：8767 / 8769

页数：3

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