Analytical methods for amino acid determination in organisms

被引:42
作者
Xu, Weihua [1 ,2 ,3 ,4 ]
Zhong, Congcong [1 ]
Zou, Chunpu [5 ]
Wang, Bing [2 ,3 ]
Zhang, Ning [1 ]
机构
[1] Shanghai Univ Tradit Chinese Med, Expt Ctr Sci & Technol, 1200 Cai Lun Rd, Shanghai 201203, Peoples R China
[2] Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, 501 Hai Ke Rd, Shanghai 201203, Peoples R China
[3] Chinese Acad Sci, Ctr Pharmaceut Res, Shanghai Inst Mat Med, 501 Hai Ke Rd, Shanghai 201203, Peoples R China
[4] Anhui Univ Tradit Chinese Med, Sch Pharm, Hefei 230013, Anhui, Peoples R China
[5] Shanghai Univ Tradit Chinese Med, Sch Basic Med, Shanghai 201203, Peoples R China
关键词
Amino acids; Analytical method; Derivation; Liquidity; MASS-SPECTROMETRY METHOD; PERFORMANCE LIQUID-CHROMATOGRAPHY; LC-MS/MS METHOD; CAPILLARY-ELECTROPHORESIS; QUANTITATIVE-ANALYSIS; PRECOLUMN DERIVATIZATION; CHIRAL ANALYSIS; HUMAN PLASMA; HUMAN SERUM; ENANTIOSELECTIVE DISCRIMINATION;
D O I
10.1007/s00726-020-02884-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Amino acids are important metabolites for tissue metabolism, growth, maintenance, and repair, which are basic life necessities. Therefore, summarizing analytical methods for amino acid determination in organisms is important. In the past decades, analytical methods for amino acids have developed rapidly but have not been fully explored. Thus, this article provides reference to analytical methods for amino acids in organisms for food and human research. Present amino acid analysis methods include thin-layer chromatography, high-performance liquid chromatography, liquid chromatography-mass spectrometer, gas chromatography-mass spectrometry, capillary electrophoresis, nuclear magnetic resonance, and amino acid analyzer analysis.
引用
收藏
页码:1071 / 1088
页数:18
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