PKU-β/TLK1 regulates myosin II activities, and is required for accurate equaled chromosome segregation

被引：12

作者：

Hashimoto, Mitsumasa ^{[1
]}

Matsui, Tadashi ^{[1
]}

Iwabuchi, Kuniyoshi ^{[1
]}

Date, Takayasu ^{[1
]}

机构：

[1] Kanazawa Med Univ, Dept Biochem, Uchinada, Ishikawa 9200293, Japan

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2008年 / 657卷 / 01期

关键词：

PKU-beta/TLK1; Myosin II; Chromosome segregation;

D O I：

10.1016/j.mrgentox.2008.09.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tousled-like kinase 1 (or protein kinase ubiquitous, PKU-beta/TLK1) is a serine/threonine protein kinase that is implicated in chromatin remodeling, DNA replication and mitosis. RNAi-mediated PKU-beta/TLK1-depleted human cells showed aneuploidy, and immunofluorescence analysis of these cells revealed the unequal segregation of daughter chromosomes. Immunoblots indicated a substantial reduction in the phosphorylation level of Ser19/Thr18 on the myosin II regulatory light chain (MRLC) in PKU-beta/TLK1-depleted cells, with no change in total MRLC protein. To confirm the relationship between mitotic aberration and MRLC dysfunction, we expressed wild type MRLC or DD-MRLC (mimics diphosphorylation; substitution of both Thr18 and Ser19 with aspartate) in PKU-beta/TLK1-depleted cells. DD-MRLC expression dramatically reduced the unequal segregation of chromosomes. Our data suggest that human PKU-beta/TLK1 plays an important role in chromosome integrity via the regulation of myosin II dynamics by phosphorylating MRLC during mitosis. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：63 / 67

页数：5

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