Importance of annealing times in the PCR amplification of GC-rich genes

被引：0
|
作者：
Subramanian, Anu [1 ]
Mamedov, Tarlan [1 ]
Pinnear, Elsje [1 ]
Viljoen, Hendrik [1 ]
机构：
[1] Univ Nebraska, Lincoln, NE USA
来源：
JOURNAL OF BIOTECHNOLOGY | 2008年 / 136卷
关键词：
D O I：
10.1016/j.jbiotec.2008.07.227
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
I4-O-003
引用
收藏
页码：S100 / S100
页数：1
相关论文
共 50 条
  • [21] Novel sulfoxides facilitate GC-rich template amplification
    Chakrabarti, R
    Schutt, CE
    BIOTECHNIQUES, 2002, 32 (04) : 866 - +
  • [22] Successful amplification of extremely GC-rich promoter regions using a novel 'slowdown PCR' technique
    Bachmann, HS
    Siffert, W
    Frey, UH
    PHARMACOGENETICS, 2003, 13 (12): : 759 - 766
  • [23] Robust PCR amplification of GC-rich targets with Hot Start 7-deaza-dGTP
    Shore, Sabrina
    Paul, Natasha
    BIOTECHNIQUES, 2010, 49 (05) : 841 - 843
  • [24] Identification of GC-rich LAT genes in birds
    Janusova, Sarka
    Krchlikova, Veronika
    Hron, Tomas D.
    Elleder, Daniel D.
    Stepanek, Ondrej D.
    PLOS ONE, 2023, 18 (04):
  • [25] PCR AMPLIFICATION OF GC-RICH TEMPLATES CONTAINING PALINDROMIC SEQUENCES USING INITIAL ALKALI DENATURATION
    CUSI, MG
    CIOE, L
    ROVERA, G
    BIOTECHNIQUES, 1992, 12 (04) : 502 - &
  • [26] Protocol for amplification of GC-rich sequences from Pseudomonas aeruginosa
    Raychaudhuri, A
    Tipton, PA
    BIOTECHNIQUES, 2004, 37 (05) : 752 - +
  • [27] Enhanced amplification of GC-rich DNA with two organic reagents
    Zhang, Zhizhou
    Yang, Xia
    Meng, Liangyu
    Liu, Fang
    Shen, Cenchao
    Yang, Wenjuan
    BIOTECHNIQUES, 2009, 47 (03) : 775 - 778
  • [28] 2-STEP HOT PCR AMPLIFICATION OF GC-RICH AVIAN C-MYC SEQUENCES
    SCHUCHARD, M
    SARKAR, G
    RUESINK, T
    SPELSBERG, TC
    BIOTECHNIQUES, 1993, 14 (03) : 390 - &
  • [29] Reliable fusion PCR mediated by GC-rich overlap sequences
    Cha-aim, Kamonchai
    Fukunaga, Tomoaki
    Hoshida, Hisashi
    Akada, Rinji
    GENE, 2009, 434 (1-2) : 43 - 49
  • [30] Effect of instrument-specific PCR cycling characteristics on amplification efficiency of a GC-rich ApoE sequence.
    Ryan, DH
    Nuccie, BL
    Yang, XH
    AMERICAN JOURNAL OF PATHOLOGY, 1996, 149 (05) : G15 - G15
12345