Assay of humoral immunity to mumps virus

被引：37

作者：

Pipkin, PA ^{[1
]}

Afzal, MA ^{[1
]}

Heath, AB ^{[1
]}

Minor, PD ^{[1
]}

机构：

[1] Natl Inst Biol Stand & Controls, Div Virol, Potters Bar EN6 3QG, Herts, England

来源：

JOURNAL OF VIROLOGICAL METHODS | 1999年 / 79卷 / 02期

关键词：

mumps virus; plaque reduction; microtitre neutralisation assay; humoral immunity;

D O I：

10.1016/S0166-0934(99)00019-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

One hundred randomly chosen sera from blood donors from North London were assayed for antibodies to mumps virus by plaque reduction and microtitre neutralisation assay, haemagglutination inhibition and in-house ELISA. The assay reproducibility was determined, and there was reasonable agreement between antibody levels measured by the two neutralising methods. Neutralising antibody levels measured by either method were low but the strain used in the assay had a large effect on the antibody titres observed. Titres measured by neutralisation assay, HI assay and ELISA did not correlate well. Assessment of immunity to mumps virus remains problematical. (C) 1999 Elsevier Science B.V. All Fights reserved.

引用

页码：219 / 225

页数：7

共 15 条

[1] Clustering of mumps virus isolates by SH gene sequence only partially reflects geographical origin [J].

Afzal, MA ;

Buchanan, J ;

Heath, AB ;

Minor, PD .

ARCHIVES OF VIROLOGY, 1997, 142 (02) :227-238

[2] MEASLES ANTIBODY - REEVALUATION OF PROTECTIVE TITERS [J].

CHEN, RT ;

MARKOWITZ, LE ;

ALBRECHT, P ;

STEWART, JA ;

MOFENSON, LM ;

PREBLUD, SR ;

ORENSTEIN, WA .

JOURNAL OF INFECTIOUS DISEASES, 1990, 162 (05) :1036-1042

[3] METHODS FOR SCREENING THE NATURALLY ACQUIRED AND VACCINE-INDUCED IMMUNITY TO THE MUMPS-VIRUS [J].

CHRISTENSON, B ;

BOTTIGER, M .

BIOLOGICALS, 1990, 18 (03) :213-219

[4]

Dias J.A., 1996, Euro Surveill, V1, P25

[5] THE RELATIONSHIP BETWEEN DOSE AND RESPONSE OF STANDARD MEASLES-VACCINES [J].

DIAZORTEGA, JL ;

FORSEY, T ;

CLEMENTS, CJ ;

MILSTIEN, J .

BIOLOGICALS, 1994, 22 (01) :35-44

[6]

FEDOVA D, 1987, Journal of Hygiene Epidemiology Microbiology and Immunology (Prague), V31, P409

[7]

HAMMON WM, 1953, JAMA-J AM MED ASSOC, V151, P1272

[8] MARMOSET LYMPHOBLASTOID-CELLS AS A SENSITIVE HOST FOR ISOLATION OF MEASLES-VIRUS [J].

KOBUNE, F ;

SAKATA, H ;

SUGIURA, A .

JOURNAL OF VIROLOGY, 1990, 64 (02) :700-705

[9]

LEINIKKI PO, 1979, P SOC EXP BIOL MED, V160, P363, DOI 10.3181/00379727-160-40451

[10] Characterization of genotype-specific epitopes of the HN protein of mumps virus [J].

Orvell, C ;

Alsheikhly, AR ;

Kalantari, M ;

Johansson, B .

JOURNAL OF GENERAL VIROLOGY, 1997, 78 :3187-3193

← 1 2 →