One-step preparation of alkyl β-D-glucosaminide by the transglycosylation of chitosan and alcohol using purified exo-β-D-glucosaminidase

被引：14

作者：

Matsumura, S ^{[1
]}

Yao, E ^{[1
]}

Toshima, K ^{[1
]}

机构：

[1] Keio Univ, Fac Sci & Technol, Dept Appl Chem, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 05期

关键词：

alkyl beta-D-glucosaminide; chitosan; enzymatic synthesis; exo-beta-D-glucosaminidase; glycoside;

D O I：

10.1023/A:1005598722617

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Alkyl beta-D-glucosaminides (RGlcN) were prepared by the novel direct transglycosylation reaction of chitosan and alcohol using the purified exo-beta-D-glucosaminidase from the chitosan-assimilating strain, Penicillium funiculosum KY616. Using the exo-beta-D-glucosaminidase, versatile synthetic intermediates, short and medium chain RGlcNs, were prepared in a maximum yield of higher than 480 mg/g-chitosan when chitosan and an alcohol were incubated at a temperature of 30 degrees C for 2 d.

引用

页码：451 / 456

页数：6

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