Evaluation of two blood microsampling approaches for drug discovery PK studies in rats

被引：25

作者：

Luo, Yongyi ^{[1
]}

Korfmacher, Walter ^{[1
]}

Ho, Stacy ^{[1
]}

Shen, Liduo ^{[1
]}

Wang, Jie ^{[1
]}

Wu, Zhongtao ^{[1
]}

Guo, Yang ^{[1
]}

Snow, Gregory ^{[2
]}

O'Shea, Thomas ^{[1
]}

机构：

[1] Drug Metab & Pharmacokinet Sanofi, Waltham, MA 02451 USA

[2] Agilux, Worcester, MA 01605 USA

来源：

BIOANALYSIS | 2015年 / 7卷 / 18期

关键词：

QUANTITATIVE BIOANALYSIS; PLASMA; SAMPLES; HEMATOCRIT; VALIDATION;

D O I：

10.4155/bio.15.126

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Serial sampling in discovery rat PK studies could be performed via capillary microsampling (CMS) of blood or by using the Mitra device to collect dried blood samples. Results: Blood CMS results were compared with Mitra sampling results for four test compounds dosed in rat PK studies. The PK profiles obtained from CMS sampling were found to be very similar to those obtained from the Mitra sampling. For 15-mu l blood CMS samples, freezing before the dilution step was found to be acceptable. Conclusion: Blood CMS using 15-mu l glass capillary microsamples works well for serial blood sampling in rat PK studies. The Mitra microsampling device provides an alternative method for collecting 10 mu l of blood as a dried blood sample.

引用

页码：2345 / 2359

页数：15

共 20 条

[1] Overcoming the barriers to the uptake of nonclinical microsampling in regulatory safety studies
Chapman, Kathryn
Chivers, Simon
Gliddon, Dan
Mitchell, David
Robinson, Sally
Sangster, Tim
Sparrow, Susan
Spooner, Neil
Wilson, Amanda
[J]. DRUG DISCOVERY TODAY, 2014, 19 (05) : 528 - 532
[2] The effect of hematocrit on bioanalysis of DBS: results from the EBF DBS-microsampling consortium
de Vries, Ronald
Barfield, Matthew
van de Merbel, Nico
Schmid, Bernhard
Siethoff, Christoph
Ortiz, Jordi
Verheij, Elwin
van Baar, Ben
Cobb, Zoe
White, Steve
Timmerman, Philip
[J]. BIOANALYSIS, 2013, 5 (17) : 2147 - 2160
[3] Quantitative bioanalysis of paracetamol in rats using volumetric absorptive microsampling (VAMS)
Denniff, Philip
Parry, Simon
Dopson, Wesley
Spooner, Neil
[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2015, 108 : 61 - 69
[4] Volumetric Absorptive Microsampling: A Dried Sample Collection Technique for Quantitative Bioanalysis
Denniff, Philip
Spooner, Neil
[J]. ANALYTICAL CHEMISTRY, 2014, 86 (16) : 8489 - 8495
[5] The effect of hematocrit on assay bias when using DBS samples for the quantitative bioanalysis of drugs
Denniff, Philip
Spooner, Neil
[J]. BIOANALYSIS, 2010, 2 (08) : 1385 - 1395
[6] A good practice guide to the administration of substances and removal of blood, including routes and volumes
Diehl, KH
Hull, R
Morton, D
Pfister, R
Rabemampianina, Y
Smith, D
Vidal, JM
van de Vorstenbosch, C
[J]. JOURNAL OF APPLIED TOXICOLOGY, 2001, 21 (01) : 15 - 23
[7] Dillen L, 2014, BIOANALYSIS, V6, P293, DOI [10.4155/BIO.13.286, 10.4155/bio.13.286]
[8] Hui Yu-Hua, 2007, Journal of Pharmacological and Toxicological Methods, V56, P256, DOI 10.1016/j.vascn.2007.05.006
[9] Jonsson O, 2013, BIOANALYSIS, V5, P449, DOI [10.4155/BIO.12.337, 10.4155/bio.12.337]
[10] Jonsson O, 2012, BIOANALYSIS, V4, P1989, DOI [10.4155/BIO.12.177, 10.4155/bio.12.177]

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