In recent years, many nanomaterials-assisted chemiluminescence (CL) systems have been developed to improve the sensitivity and to expand the scope of their analytical applications. In these new systems, nanomaterials participate in CL reactions as catalysts, labels, reductants, luminophors, or energy acceptors. This review mainly focuses on the recent analytical applications of metal nanoparticles, magnetic nanoparticles, quantum dots (QDs), and carbon-based nanomaterials (carbon nanotubes and graphene) in liquid-phase CL systems. Recent advances in electrochemiluminescence based on nanotechnology and its analytical applications, especially in immunoassay, DNA analysis, and other biological analyses, are also summarized. Finally, we discuss some critical challenges in this field and speculate about their solutions. A total of 177 references mainly in the last 5years are included in this review.