Interaction of Ru(phen)3Cl2 with graphene oxide and its application for DNA detection both in vitro and in vivo

被引：33

作者：

Li, Haiju ^{[1
]}

Liu, Fengyu ^{[2
]}

Sun, Shiguo ^{[1
]}

Wang, Jingyun ^{[3
]}

Li, Zhiyong ^{[1
]}

Mu, Daozhou ^{[1
]}

Qiao, Bo ^{[1
]}

Peng, Xiaojun ^{[1
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China

[2] Dalian Univ Technol, Sch Chem, Dalian 116023, Peoples R China

[3] Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2013年 / 1卷 / 33期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

CARBON NANOTUBES; COMPLEXES; BINDING; RUTHENIUM(II); FILMS; TRIS(PHENANTHROLINE)RUTHENIUM(II); FUNCTIONALIZATION; PHOTOPHYSICS; CHEMISTRY; DELIVERY;

D O I：

10.1039/c3tb20858c

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

The fluorescence of Ru(phen)(3)Cl-2 (tris(1,10-phenanthroline) ruthenium(II) dichloride) can be effectively quenched with the addition of graphene oxide in aqueous solution, due to the formation of a GO-Ru hybrid. A fluorescence enhancement of approximately 50 times can be observed after the addition of a certain amount of DNA into the above-mentioned solution. The fluorescence increase is linearly proportional to the amount of DNA added in the concentration range of 0-70 mu M and the DNA detection limit is down to 3.3 x 10(-8) M. Meanwhile, it is found that the GO-Ru hybrid can enter into the nuclei and stain the DNA of living human breast cancer cells MCF-7, while Ru(phen)(3)Cl-2 alone cannot cross the cellular membrane in the control experiment. This method can be employed to detect DNA both in vitro and in vivo.

引用

页码：4146 / 4151

页数：6

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