Preparation of ADM/PRP freeze-dried dressing and effect of mice full-thickness skin defect model

被引：26
作者：
Lei, Xiaoxuan [1 ,2 ]
Yang, Yu [1 ,2 ]
Shan, Guiqiu [3 ]
Pan, Yingen [4 ]
Cheng, Biao [1 ,2 ]
机构：
[1] Southern Med Univ, Grad Sch, Guangzhou 510515, Guangdong, Peoples R China
[2] Guangzhou Gen Hosp Guangzhou Mil Command, Ctr Wound Treatment, Guangzhou 510010, Guangdong, Peoples R China
[3] Guangzhou Gen Hosp Guangzhou Mil Command, Dept Blood Transfus, Guangzhou 510010, Guangdong, Peoples R China
[4] Qidong Peoples Hosp, Dept Burn & Plast Surg, Qidong 226200, Peoples R China
来源：
BIOMEDICAL MATERIALS | 2019年 / 14卷 / 03期
基金：
中国国家自然科学基金;
关键词：
platelet-rich plasma; freeze-dried platelet-rich plasma; acellular dermal matrix; acute wounds; ACELLULAR DERMAL MATRIX; PLATELET-RICH PLASMA; COMPONENT SEPARATION; STEM-CELLS; REPAIR; SCAFFOLDS;
D O I：
10.1088/1748-605X/ab0060
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Acellular dermal matrix (ADM) and platelet-rich plasma (PRP) have each been used in wound healing. There are a few reports on the application of ADM with PRP in skin full-thickness defect models. In this study, the microstructure of ADM/PRP freeze-dried dressing was observed by scanning electron microscope. The PRP, ADM and PRP/ADM samples were recorded by Fourier transform infrared (FTIR) spectrometer by the KBr methods. The concentration of growth factor was measured by enzyme-linked immunosorbent assay. Then, we made a mice full-thickness defect model and treated it with saline, PRP, ADM and PRP/ADM, respectively. The wound size was measured and calculated on days 3, 5, 7, 10 and 14. The obtained results demonstrated that the ADM/PRP freeze-dried dressing resulted in earlier collagen development. FTIR analysis confirmed the integration of ADM and PRP in the prepared PRP/ADM scaffold. The concentration of bFGF, EGF, TGF-beta 1 and PDGF-BB in the ADM/PRP group was lower than that in the PRP group. The wound healing rate of the ADM/PRP group was significantly promoted. Furthermore, the ADM/PRP group had significant revascularization, rapid epithelialization and a well-differentiated epidermis. The collagen fibers were regularly arranged. Accordingly, ADM/PRP freeze-dried dressing promotes wound healing and can be used in the skin full-thickness wound.
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页数：12
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