Electrochemical sensing of label free DNA hybridization related to breast cancer 1 gene at disposable sensor platforms modified with single walled carbon nanotubes

被引：55
作者：
Li, Chen-zhong [1 ,2 ]
Karadeniz, Hakan [1 ]
Canavar, Ece [1 ]
Erdem, Arzum [1 ]
机构：
[1] Ege Univ, Fac Pharm, Dept Analyt Chem, TR-35100 Izmir, Turkey
[2] Florida Int Univ, Dept Biomed Engn, Nanobioengn Bioelect Lab, Miami, FL 33199 USA
来源：
ELECTROCHIMICA ACTA | 2012年 / 82卷
基金：
美国国家科学基金会;
关键词：
Screen printed graphite electrode; Carbon nanotubes; DNA hybridization; Electrochemical sensor; BRCA1; Electrochemical impedance spectroscopy; GRAPHITE-ELECTRODES; NUCLEIC-ACIDS; BIOSENSORS;
D O I：
10.1016/j.electacta.2012.05.057
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
The electrochemical monitoring of DNA hybridization related to specific sequences on breast cancer 1 (BRCA1) DNA by using singlewalled carbon nanotube (SWCNT) based screen printed graphite electrodes (SPEs) was performed in this study. After the microscopic characterization of SWCNT-SPE, the optimization of assay was studied. The development of screen printing process combined with nanomaterial based disposable sensor technology with sensitivity, selectivity and reproducibility, has a great opportunity for DNA detection using differential pulse voltammetry (DPV) by measuring the guanine oxidation signal observed at +1.00 V in the presence of DNA hybridization between BRCA1 probe and its complementary target. The detection limit estimated for signal to noise ratio = 3 corresponds to 378.52 nM target concentration in the 401,1 samples. The voltammetric results on BRCA1 DNA hybridization were also complemented with electrochemical impedance spectroscopy (EIS). (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：137 / 142
页数：6
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