Mechanical Insight into Resistance of Betaine to Urea-Induced Protein Denaturation

被引：12

作者：

Chen, Jiantao ^{[1
]}

Gong, Xiangjun ^{[1
]}

Zeng, Chaoxi ^{[2
]}

Wang, Yonghua ^{[2
]}

Zhang, Guangzhao ^{[1
]}

机构：

[1] South China Univ Technol, Fac Mat Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China

[2] South China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2016年 / 120卷 / 48期

基金：

中国国家自然科学基金;

关键词：

NUCLEAR-MAGNETIC-RESONANCE; MOLECULAR-DYNAMICS SIMULATION; TRIMETHYLAMINE N-OXIDE; GLYCINE BETAINE; GUANIDINE-HYDROCHLORIDE; AQUEOUS-SOLUTIONS; ALPHA-CHYMOTRYPSIN; STRUCTURE BREAKER; WATER; BEHAVIOR;

D O I：

10.1021/acs.jpcb.6b10172

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

It is known that urea can induce protein denaturation that can be inhibited by osmolytes. Yet, experimental explorations on this mechanism at the molecular level are still lacking. We have investigated the resistance of betaine to the urea-induced denaturation of lysozyme in aqueous solutions using low-field NMR. Our study demonstrates that urea molecules directly interact with lysozyme, leading to denaturation. However, betaine molecules interacting with urea more strongly than lysozyme can pull the bound urea molecules from lysozyme so that the protein is protected from denaturation. The number of urea molecules bound to a betaine molecule is given under different conditions. Proton NMR spectroscopy (H-1-NMR) and Fourier transform infrared spectroscopy reveal that the interaction between betaine and urea is through hydrogen bonding.

引用

页码：12327 / 12333

页数：7

共 49 条

[11] PROTON-TRANSFER STUDIES BY NUCLEAR MAGNETIC RESONANCE .1. DIFFUSION CONTROL IN THE REACTION OF AMMONIUM ION IN AQUEOUS ACID [J].

EMERSON, MT ;

GRUNWALD, E ;

KROMHOUT, RA .

JOURNAL OF CHEMICAL PHYSICS, 1960, 33 (02) :547-555

[12] The exclusion of glycine betaine from anionic biopolymer surface: Why glycine betaine is an effective osmoprotectant but also a compatible solute [J].

Felitsky, DJ ;

Cannon, JG ;

Capp, MW ;

Hong, J ;

Van Wynsberghe, AW ;

Anderson, CF ;

Record, MT .

BIOCHEMISTRY, 2004, 43 (46) :14732-14743

[13] NUCLEAR MAGNETIC-RESONANCE STUDIES OF AQUEOUS UREA SOLUTIONS [J].

FINER, EG ;

FRANKS, F ;

TAIT, MJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1972, 94 (13) :4424-&

[14] STRUCTURAL APPROACH TO SOLVENT POWER OF WATER FOR HYDROCARBONS - UREA AS A STRUCTURE BREAKER [J].

FRANK, HS ;

FRANKS, F .

JOURNAL OF CHEMICAL PHYSICS, 1968, 48 (10) :4746-&

[15] COMPATIBLE SOLUTES OF HALOPHILIC EUBACTERIA - MOLECULAR PRINCIPLES, WATER-SOLUTE INTERACTION, STRESS PROTECTION [J].

GALINSKI, EA .

EXPERIENTIA, 1993, 49 (6-7) :487-496

[16] Critical Effect of Segmental Dynamics in Polybutadiene/Clay Nanocomposites Characterized by Solid State 1H NMR Spectroscopy [J].

Gao, Yun ;

Zhang, Rongchun ;

Lv, Weifeng ;

Liu, Qingjie ;

Wang, Xiaoliang ;

Sun, Pingchuan ;

Winter, H. Henning ;

Xue, Gi .

JOURNAL OF PHYSICAL CHEMISTRY C, 2014, 118 (10) :5606-5614

[17]

GREENE RF, 1974, J BIOL CHEM, V249, P5388

[18] Quantifying why urea is a protein denaturant, whereas glycine betaine is a protein stabilizer [J].

Guinn, Emily J. ;

Pegram, Laurel M. ;

Capp, Michael W. ;

Pollock, Michelle N. ;

Record, M. Thomas, Jr. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (41) :16932-16937

[19] COOPERATIVITY OF SOLVENT-MACROMOLECULE INTERACTIONS IN AQUEOUS SOLUTIONS OF POLYETHYLENE GLYCOL AND POLYETHYLENE GLYCOL-UREA [J].

HAMMES, GG ;

ROBERTS, PB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1968, 90 (25) :7119-&

[20] THE EFFECTS OF PROTEINS ON THE PROTON NMR TRANSVERSE RELAXATION-TIME OF WATER .2. PROTEIN AGGREGATION [J].

HILLS, BP ;

TAKACS, SF ;

BELTON, PS .

MOLECULAR PHYSICS, 1989, 67 (04) :919-937

← 1 2 3 4 5 →