Lack of genomic imprinting of DNA primase, polypeptide 2 (PRIM2) in human term placenta and white blood cells

被引：8

作者：

Chung, Jaewook ^{[1
]}

Tsai, Shengdar ^{[1
]}

James, Andra H. ^{[2
]}

Thames, Betty H. ^{[2
]}

Shytle, Stephanie ^{[2
]}

Piedrahita, Jorge A. ^{[1
]}

机构：

[1] N Carolina State Univ, Ctr Comparat Med & Translat Res, Raleigh, NC 27695 USA

[2] Duke Univ, Sch Med, Dept Obstet & Gynecol, Durham, NC USA

来源：

EPIGENETICS | 2012年 / 7卷 / 05期

关键词：

genomic imprinting; PRIM2; pseudogene; placenta; WBC; SNP; sequencing; P58; SUBUNIT; GENES; MOUSE; REPLICATION; INITIATION; EXPRESSION; ORIGIN;

D O I：

10.4161/epi.19777

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PRIM2, encoding a subunit of primase involved in DNA replication and transcription, is expressed in the placenta and is crucial for mammalian development and growth. Its role in placental function is not well understood. Recently, PRIM2 was reported as imprinted in human white blood cells (WBC). We report here our failure to confirm imprinting of the PRIM2 locus in human placenta or WBC. The discordance between our results and those of others are likely due to an incorrectly annotated PRIM2 pseudogene found in the human genome database.

引用

页码：429 / 431

页数：3

共 20 条

[1]

COPELAND WC, 1993, J BIOL CHEM, V268, P26179

[2] Genomic imprinting: Implications for human disease [J].

Falls, JG ;

Pulford, DJ ;

Wylie, AA ;

Jirtle, RL .

AMERICAN JOURNAL OF PATHOLOGY, 1999, 154 (03) :635-647

[3] The imprinted gene and parent-of-origin effect database now includes parental origin of de novo mutations [J].

Glaser, Rivka L. ;

Ramsay, Joshua P. ;

Morison, Ian M. .

NUCLEIC ACIDS RESEARCH, 2006, 34 :D29-D31

[4] High-Resolution Analysis of Parent-of-Origin Allelic Expression in the Mouse Brain [J].

Gregg, Christopher ;

Zhang, Jiangwen ;

Weissbourd, Brandon ;

Luo, Shujun ;

Schroth, Gary P. ;

Haig, David ;

Dulac, Catherine .

SCIENCE, 2010, 329 (5992) :643-648

[5] The Genomic Distribution and Local Context of Coincident SNPs in Human and Chimpanzee [J].

Hodgkinson, Alan ;

Eyre-Walker, Adam .

GENOME BIOLOGY AND EVOLUTION, 2010, 2 :547-557

[6] Computational and experimental identification of novel human imprinted genes [J].

Luedi, Philippe P. ;

Dietrich, Fred S. ;

Weidman, Jennifer R. ;

Bosko, Jason M. ;

Jirtle, Randy L. ;

Hartemink, Alexander J. .

GENOME RESEARCH, 2007, 17 (12) :1723-1730

[7] Genome-wide prediction of imprinted murine genes [J].

Luedi, PP ;

Hartemink, AJ ;

Jirtle, RL .

GENOME RESEARCH, 2005, 15 (06) :875-884

[8] Epigenetics in the Placenta [J].

Maccani, Matthew A. ;

Marsit, Carmen J. .

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2009, 62 (02) :78-89

[9] COMPLETION OF MOUSE EMBRYOGENESIS REQUIRES BOTH THE MATERNAL AND PATERNAL GENOMES [J].

MCGRATH, J ;

SOLTER, D .

CELL, 1984, 37 (01) :179-183

[10] Limited evolutionary conservation of imprinting in the human placenta [J].

Monk, D ;

Arnaud, P ;

Apostolidou, S ;

Hills, FA ;

Kelsey, G ;

Stanier, P ;

Feil, R ;

Moore, GE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (17) :6623-6628

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