Genome-wide analysis and expression profiling of the Solanum tuberosum aquaporins

被引：77

作者：

Venkatesh, Jelli ^{[1
]}

Yu, Jae-Woong ^{[1
]}

Park, Se Won ^{[1
]}

机构：

[1] Konkuk Univ, Dept Mol Biotechnol, Seoul, South Korea

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2013年 / 73卷

关键词：

Abiotic stress; Aquaporin; Homologues; Major intrinsic proteins; Phylogenetics; Solanum tuberosum; MAJOR INTRINSIC PROTEINS; PLASMA-MEMBRANE AQUAPORINS; MULTIPLE SEQUENCE ALIGNMENT; GENE FAMILY; WATER CHANNELS; ARABIDOPSIS; SELECTIVITY; STRESS; IDENTIFICATION; TRANSPORT;

D O I：

10.1016/j.plaphy.2013.10.025

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Aquaporins belongs to the major intrinsic proteins involved in the transcellular membrane transport of water and other small solutes. A comprehensive genome-wide search for the homologues of Solanum tuberosum major intrinsic protein (MIP) revealed 41 full-length potato aquaporin genes. All potato aquaporins are grouped into five subfamilies; plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), NOD26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs) and x-intrinsic proteins (XIPs). Functional predictions based on the aromatic/arginine (ar/R) selectivity filters and Froger's positions showed a remarkable difference in substrate transport specificity among subfamilies. The expression pattern of potato aquaporins, examined by qPCR analysis, showed distinct expression profiles in various organs and tuber developmental stages. Furthermore, qPCR analysis of potato plantlets, subjected to various abiotic stresses revealed the marked effect of stresses on expression levels of aquaporins. Taken together, the expression profiles of aquaporins imply that aquaporins play important roles in plant growth and development, in addition to maintaining water homeostasis in response to environmental stresses. (C) 2013 Elsevier Masson SAS. All rights reserved.

引用

页码：392 / 404

页数：13

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