Fluorescence detection of cationic amyloid fibrils in human semen

被引：11

作者：

Easterhoff, David ^{[1
]}

DiMaio, John T. M. ^{[2
]}

Liyanage, Wathsala ^{[2
]}

Lo, Chi-Wen ^{[3
]}

Bae, Woori ^{[1
]}

Doran, Todd M. ^{[2
]}

Smrcka, Alan ^{[4
]}

Nilsson, Bradley L. ^{[2
]}

Dewhurst, Stephen ^{[1
]}

机构：

[1] Univ Rochester, Dept Microbiol & Immunol, Rochester, NY 14642 USA

[2] Univ Rochester, Dept Chem, Rochester, NY 14627 USA

[3] Univ Rochester, Dept Pathol & Lab Med, Rochester, NY 14642 USA

[4] Univ Rochester, Dept Physiol & Pharmacol, Rochester, NY 14642 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2013年 / 23卷 / 18期

关键词：

Amyloid; Thioflavin T; Semen; SEVI; Fluorescence; ENHANCE HIV-INFECTION; THIOFLAVIN-T; MEDIATED ENHANCEMENT; SEVI; EGCG; PEPTIDES; PLAQUES; BINDING; PROBES; BETA;

D O I：

10.1016/j.bmcl.2013.06.097

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Cationic amyloid fibrils, including the Semen Enhancer of Virus Infection (SEVI), have recently been described in human semen. Simple methods for quantitating these fibrils are needed to improve our understanding of their biological function. We performed high-throughput screening to identify molecules that bind SEVI, and identified a small molecule (8E2), that fluoresced brightly in the presence of SEVI and other cationic fibrils. 8E2 bound SEVI with almost 40-fold greater affinity than thioflavin-T, and could efficiently detect high molecular weight fibrils in human seminal fluid. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.

引用

页码：5199 / 5202

页数：4

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