Cloning of the RhoB gene from the mouse genome and characterization of its promoter region

被引：9
作者：
Nakamura, T
Asano, M
ShindoOkada, N
Nishimura, S
Monden, Y
机构：
[1] MERCK RES LABS,BANYU TSUKUBA RES INST,TSUKUBA,IBARAKI 30026,JAPAN
[2] NATL CANC CTR,RES INST,DIV BIOL,CHUO KU,TOKYO 104,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 226卷 / 03期
关键词：
D O I：
10.1006/bbrc.1996.1415
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Rho proteins have been implicated in a variety of cytoskeletal functions, but it is unclear how Rho proteins regulate these cellular functions and how Rho proteins are regulated. In this study, we cloned the rhoB gene from the mouse genome and characterized its promoter region. The predicted amino acid sequence was identical to that encoded by the human rhoB gene. A site for initiation of transcription was found al position -376 relative to the site for initiation of translation. Deletion analysis of the 5'-flanking region of the rhoB gene revealed that the minimum region of the promoter was located between positions -507 and -376. Northern blotting analysis showed that the expression of the mouse rhoB gene was induced by serum, suggesting that expression of the rhoB gene might be controlled by some signal-responsive element(s). (C) 1996 Academic Press, Inc.
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页码：688 / 694
页数：7
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