Large sub-clonal variation in Phytophthora infestans from recent severe late blight epidemics in India

被引:50
作者
Dey, Tanmoy [1 ]
Saville, Amanda [2 ]
Myers, Kevin [3 ]
Tewari, Susanta [4 ]
Cooke, David E. L. [5 ]
Tripathy, Sucheta [6 ,7 ]
Fry, William E. [3 ]
Ristaino, Jean B. [2 ]
Roy, Sanjoy Guha [1 ]
机构
[1] West Bengal State Univ, Dept Bot, Kolkata 700126, India
[2] North Carolina State Univ, Dept Entomol & Plant Pathol, Raleigh, NC 27607 USA
[3] Cornell Univ, Plant Pathol & Plant Microbe Biol, Ithaca, NY 14850 USA
[4] TeraBundle Anlyt Pvt Ltd, Haldia 721602, India
[5] James Hutton Inst, Dundee DD2 5DA, Scotland
[6] Indian Inst Chem Biol, Kolkata 700032, India
[7] Acad Sci & Innovat Res, Delhi, India
关键词
POTATO FAMINE PATHOGEN; EASTERN UNITED-STATES; POPULATION-STRUCTURE; GENOTYPIC DIVERSITY; PCR AMPLIFICATION; SEVERE OUTBREAKS; LINEAGE; TOMATO; SEQUENCE; DNA;
D O I
10.1038/s41598-018-22192-1
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The population structure of the Phytophthora infestans populations that caused the recent 2013-14 late blight epidemic in eastern India (EI) and northeastern India (NEI) was examined. The data provide new baseline information for populations of P. infestans in India. A migrant European 13_A2 genotype was responsible for the 2013-14 epidemic, replacing the existing populations. Mutations have generated substantial sub-clonal variation with 24 multi-locus genotypes (MLGs) found, of which 19 were unique variants not yet reported elsewhere globally. Samples from West Bengal were the most diverse and grouped alongside MLGs found in Europe, the UK and from neighbouring Bangladesh but were not linked directly to most samples from south India. The pathogen population was broadly more aggressive on potato than on tomato and resistant to the fungicide metalaxyl. Pathogen population diversity was higher in regions around the international borders with Bangladesh and Nepal. Overall, the multiple shared MLGs suggested genetic contributions from UK and Europe in addition to a sub-structure based on the geographical location within India. Our data indicate the need for improved phytosanitary procedures and continuous surveillance to prevent the further introduction of aggressive lineages of P. infestans into the country.
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页数:12
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