Fluorimetric method for the determination of thiocyanate with 2′,7′-dichlorofluorescein and iodine

被引：33
作者：
Gong, BL [1 ]
Gong, GQ
机构：
[1] Ningxia Univ, Dept Chem, Yingchuan 750021, Peoples R China
[2] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 1999年 / 394卷 / 2-3期
关键词：
fluorimetry; thiocyanate; 2; 7; '-dichlorofluorescein; iodine; saliva; serum;
D O I：
10.1016/S0003-2670(99)00295-0
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A highly sensitive and selective fluorimetric method has been developed for the determination of thiocyanate with 2',7'-dichlorofluorescein (lambda(ex) = 508 nm, lambda(em) = 524 nm) at pH 5.8-6.7. The calibration gaph is linear over the range 1.0-60 ng ml(-1) SCN-. The detection limit is 1.0 ng ml(-1) SCN-. The method has been successfully applied to the determination of thiocyanate ion in saliva and serum. (C) 1999 Elsevier Science B.V. All rights reserved.
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页码：171 / 175
页数：5
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