Specific humoral and cellular immunity induced by Trypanosoma cruzi DNA immunization in a canine model

被引:24
作者
Arce-Fonseca, Minerva [1 ]
Ballinas-Verdugo, Martha A. [1 ]
Abreu Zenteno, Emma R. [1 ]
Suarez-Flores, Davinia [1 ]
Carrillo-Sanchez, Silvia C. [1 ]
Alejandre-Aguilar, Ricardo [2 ]
Luis Rosales-Encina, Jose [3 ]
Reyes, Pedro A. [4 ]
Rodriguez-Morales, Olivia [1 ]
机构
[1] Inst Nacl Cardiol Ignacio Chavez, Dept Mol Biol, Mexico City 14080, DF, Mexico
[2] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Parasitol, Mexico City 11340, DF, Mexico
[3] Ctr Invest & Estudios Avanzados IPN, Dept Infect & Mol Pathogenesis, Mexico City 07360, DF, Mexico
[4] Inst Nacl Cardiol Ignacio Chavez, Res Direct, Mexico City 14080, DF, Mexico
关键词
CHAGAS-DISEASE; LYTIC ANTIBODIES; INFECTION; VACCINE; PROTECTION; SUSCEPTIBILITY; RESPONSES; MUCOSAL; CONFERS; PROTEIN;
D O I
10.1186/1297-9716-44-15
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Chagas disease has a high incidence in Mexico and other Latin American countries. Because one of the most important known methods of prevention is vector control, which has been effective only in certain areas of South America, the development of a vaccine to protect people at risk has been proposed. In this study, we assessed the cellular and humoral immune response generated following immunization with pBCSP and pBCSSP4 plasmids containing the genes encoding a trans-sialidase protein (present in all three forms of T. cruzi) and an amastigote specific glycoprotein, respectively, in a canine model. Thirty-five beagle dogs were divided randomly into 5 groups (n = 7) and were immunized twice intramuscularly with 500 mu g of pBCSSP4, pBCSP, pBk-CMV (empty plasmid) or saline solution. Fifteen days after the last immunization the 4 groups were infected intraperitoneally with 500 000 metacyclic trypomastigotes. The fifth group was unimmunized/infected. The parasitaemia in the immunized/infected dogs was for a shorter period (14 vs. 29 days) and the parasite load was lower. The concentration of IgG1 (0.612 +/- 0.019 O.D.) and IgG2 (1.167 +/- 0.097 O.D.) subclasses was measured (absorbance) 15 days after the last immunization with both recombinant plasmids, the majority of which were IgG2. The treatment of parasites using the serum from dogs immunized with pBCSP and pBCSSP4 plasmids produced 54% (+/- 11.8) and 68% (+/- 21.4) complement-mediated lysis, respectively. At 12 h post immunization, an increase in cytokines was not observed; however, vaccination with pBCSSP4 significantly increased the levels of IFN-gamma and IL-10 at 9 months post-infection. The recombinant plasmid immunization stimulated the spleen cell proliferation showing a positive stimulatory index above 2.0. In conclusion, immunization using both genes effectively induces a humoral and cellular immune response.
引用
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页数:9
相关论文
共 36 条
[1]   Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine [J].
Amara, RR ;
Villinger, F ;
Altman, JD ;
Lydy, SL ;
O'Neil, SP ;
Staprans, SI ;
Montefiori, DC ;
Xu, Y ;
Herndon, JG ;
Wyatt, LS ;
Candido, MA ;
Kozyr, NL ;
Earl, PL ;
Smith, JM ;
Ma, HL ;
Grimm, BD ;
Hulsey, ML ;
Miller, J ;
McClure, HM ;
McNicholl, JM ;
Moss, B ;
Robinson, HL .
SCIENCE, 2001, 292 (5514) :69-74
[2]  
[Anonymous], 1999, NORM OFF MEX NOM 006
[3]   Testing the Efficacy of a Multi-Component DNA-Prime/DNA-Boost Vaccine against Trypanosoma cruzi Infection in Dogs [J].
Aparicio-Burgos, Jose E. ;
Ochoa-Garcia, Laucel ;
Antonio Zepeda-Escobar, Jose ;
Gupta, Shivali ;
Dhiman, Monisha ;
Simon Martinez, Jose ;
Montes de Oca-Jimenez, Roberto ;
Val Arreola, Margarita ;
Barbabosa-Pliego, Alberto ;
Vazquez-Chagoyan, Juan C. ;
Garg, Nisha Jain .
PLOS NEGLECTED TROPICAL DISEASES, 2011, 5 (05)
[4]   A DNA Vaccine Encoding for TcSSP4 Induces Protection against Acute and Chronic Infection in Experimental Chagas Disease [J].
Arce-Fonseca, Minerva ;
Ramos-Ligonio, Angel ;
Lopez-Monteon, Aracely ;
Salgado-Jimenez, Berenice ;
Talamas-Rohana, Patricia ;
Luis Rosales-Encina, Jose .
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2011, 7 (09) :1230-1238
[5]   DIFFERENCE IN SUSCEPTIBILITY TO LYSIS BETWEEN CLONES OF THE Y-STRAIN OF TRYPANOSOMA-CRUZI [J].
BRAGA, EM ;
GALVAO, LMC ;
CHIARI, E ;
MARTINS, MS .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1993, 88 (04) :529-534
[6]   Systemic administration of immunostimulatory DNA sequences mediates reversible inhibition of Th2 responses in a mouse model of asthma [J].
Broide, DH ;
Stachnick, G ;
Castaneda, D ;
Nayar, J ;
Miller, M ;
Cho, JY ;
Roman, M ;
Zubeldia, J ;
Hyashi, T ;
Raz, E .
JOURNAL OF CLINICAL IMMUNOLOGY, 2001, 21 (03) :175-182
[7]   Prime-boost immunization with cruzipain co-administered with MALP-2 triggers a protective immune response able to decrease parasite burden and tissue injury in an experimental Tryponosoma cruzi infection model [J].
Cazorla, Silvia I. ;
Frank, Fernanda M. ;
Becker, Pablo D. ;
Corral, Ricardo S. ;
Guzman, Carlos A. ;
Malchiodi, Emilio L. .
VACCINE, 2008, 26 (16) :1999-2009
[8]   Complement C2 receptor inhibitor trispanning confers an increased ability to resist complement-mediated lysis in Trypanosoma cruzi [J].
Cestari, Igor dos S. ;
Evans-Osses, Ingrid ;
Freitas, Juliana C. ;
Inal, Jameel M. ;
Ramirez, Marcel I. .
JOURNAL OF INFECTIOUS DISEASES, 2008, 198 (09) :1276-1283
[9]   Trypanosoma cruzi: Immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes [J].
Coura-Vital, W. ;
Carneiro, C. M. ;
Martins, H. R. ;
de lana, M. ;
Veloso, V. M. ;
Teixeira-Carvalho, A. ;
Bahia, M. T. ;
Correa-Oliveira, R. ;
Martins-Filho, O. A. ;
Tafuri, W. L. ;
Reis, A. B. .
EXPERIMENTAL PARASITOLOGY, 2008, 120 (03) :269-274
[10]  
Fossum T., 2007, Small animal surgery, V3rd