Determination of Isoelectric Points and the Role of pH for Common Quartz Crystal Microbalance Sensors

被引：71

作者：

Cuddy, Michael F. ^{[1
]}

Poda, Aimee R. ^{[1
]}

Brantley, Lauren N. ^{[1
]}

机构：

[1] USA, Environm Lab, Corps Engineer Res & Dev Ctr, Vicksburg, MS 39180 USA

来源：

ACS APPLIED MATERIALS & INTERFACES | 2013年 / 5卷 / 09期

关键词：

isoelectric point; IEP; quartz crystal microbalance; QCM; sensor; mucin; CONTACT-ANGLE TITRATIONS; PORCINE GASTRIC MUCIN; FORCE MEASUREMENTS; DOUBLE-LAYER; SURFACE; OXIDE; ADSORPTION; AGGREGATION; TRANSITION; FILMS;

D O I：

10.1021/am400909g

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Isoelectric points (IEPs) were determined by the method of contact angle titration for five common quartz crystal microbalance (QCM) sensors. The isoelectric points range from mildly basic in the case of Al2O3 sensors (IEP = 8.7) to moderately acidic for Au (5.2) and SiO2 (3.9), to acidic for Ag (3.2) and Ti (2.9). In general, the values reported here are indicative of inherent surface oxides. A demonstration of the effect of the surface isoelectric point on the packing efficiency of thin mucin films is provided for gold and silica QCM sensors. It is determined that mucin layers on both substrates achieve a maximum and equal layer density of similar to 3500 kg/m(3) at the corresponding IEP of either QCM sensor. This implies that mucin film packing is dependent upon short-range electrostatic interactions at the sensor surface.

引用

页码：3514 / 3518

页数：5

共 30 条

[1] MUCIN BIOPHYSICS [J].

BANSIL, R ;

STANLEY, HE ;

LAMONT, JT .

ANNUAL REVIEW OF PHYSIOLOGY, 1995, 57 :635-657

[2] Mucin structure, aggregation, physiological functions and biomedical applications [J].

Bansil, Rama ;

Turner, Bradley S. .

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 2006, 11 (2-3) :164-170

[3] Double layer of a gold electrode probed by AFM force measurements [J].

Barten, D ;

Kleijn, JM ;

Duval, J ;

von Leeuwen, HP ;

Lyklema, J ;

Stuart, MAC .

LANGMUIR, 2003, 19 (04) :1133-1139

[4]

Benesi H.A., 1978, ADV CATAL, V27, P97

[5] PROFOUND INCREASE IN VISCOSITY AND AGGREGATION OF PIG GASTRIC MUCIN AT LOW PH [J].

BHASKAR, KR ;

GONG, D ;

BANSIL, R ;

PAJEVIC, S ;

HAMILTON, JA ;

TURNER, BS ;

LAMONT, JT .

AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (05) :G827-G833

[6] pH-dependent conformational change of gastric mucin leads to sol-gel transition [J].

Cao, XX ;

Bansil, R ;

Bhaskar, KR ;

Turner, BS ;

LaMont, JT ;

Niu, N ;

Afdhal, NH .

BIOPHYSICAL JOURNAL, 1999, 76 (03) :1250-1258

[7] Viscoelastic properties and dynamics of porcine gastric mucin [J].

Celli, J ;

Gregor, B ;

Turner, B ;

Afdhal, NH ;

Bansil, R ;

Erramilli, S .

BIOMACROMOLECULES, 2005, 6 (03) :1329-1333

[8] SURFACE ISOELECTRIC POINT OF EVAPORATED SILVER FILMS - DETERMINATION BY CONTACT-ANGLE TITRATION [J].

CHAU, LK ;

PORTER, MD .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1991, 145 (01) :283-286

[9] The electrical double layer on gold probed by electrokinetic and surface force measurements [J].

Giesbers, M ;

Kleijn, JM ;

Stuart, MAC .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2002, 248 (01) :88-95

[10] Electrostatics and Metal Oxide Wettability [J].

Hanly, Gary ;

Fornasiero, Daniel ;

Ralston, John ;

Sedev, Rossen .

JOURNAL OF PHYSICAL CHEMISTRY C, 2011, 115 (30) :14914-14921

← 1 2 3 →