Effect of mobile phase pH* on chromatographic behaviour in chiral ligand-exchange thin-layer chromatography (CLETLC) of amino acid enantiomers

被引：15

作者：

Polak, B ^{[1
]}

Golkiewicz, W ^{[1
]}

Tuzimski, T ^{[1
]}

机构：

[1] Med Univ Lublin, Dept Chem Phys, PL-20081 Lublin, Poland

来源：

CHROMATOGRAPHIA | 2006年 / 63卷 / 3-4期

关键词：

thin-layer-chromatography; chiral ligand-exchange; mobile-phase pH; amino acids;

D O I：

10.1365/s10337-005-0681-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The enantiomers of some amino acids have been separated on commercial chiral TLC plates in reversed-phase mode. The effect of the pH* of the aqueous-organic mobile phase on the retention and mobility of the enantiomers and on selectivity was investigated. It was shown that for most of the amino acids investigated the highest enantioselectivity was obtained at pH* 3-4 or 6-7. The drift and disturbance of the baseline on the chromatograms were also much smaller at pH* 3-4 and 6-7.

引用

页码：197 / 201

页数：5

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