Ultrasound extraction optimization of Acanthopanax senticosus polysaccharides and its antioxidant activity

被引:49
|
作者
Zhao, Zhanyi [1 ]
Xu, Xiaojiang [1 ]
Ye, Qingwen [1 ]
Dong, Lulu [1 ]
机构
[1] Harbin Med Univ, Coll Pharm, Harbin 150086, Peoples R China
关键词
Ultrasound extraction; Polysaccharides; Acanthopanax senticosus; Antioxidant activity; Orthogonal test design; WATER-SOLUBLE POLYSACCHARIDES; ASSISTED EXTRACTION; CHEMISTRY; LUCIDUM; BODIES;
D O I
10.1016/j.ijbiomac.2013.04.067
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, effects of several experimental parameters, including ultrasonic time, ratio of water to material, extraction temperature and ultrasonic power on the extraction yield of polysaccharides from Acanthopanax senticosus stem (ASS) were investigated by single factor experiment and an orthogonal test design (L-9(3)(4)) was used to optimize the ultrasound extraction conditions. The polysaccharides from Acanthopanax senticosus stem (ASSP) and fruit (ASFP) were further fractionated by stepwise ethanol precipitation and the anti-oxidation activities of those fractions were evaluated by hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Under the optimal conditions (ultrasonic time 75 min, ratio of water to material 50 ml/g, extraction temperature 80 degrees C and ultrasonic power 100W), the yield was 10.9 mg/g. All fractions of ASP possessed considerable antioxidant activity. The results indicated that the ultrasound extraction was a very useful method for the extraction of ASP and the polysaccharides could be explored as a potential antioxidant agent for use in medicine or functional food. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:290 / 294
页数:5
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