Tissue injury following inhalation of fine particulate matter and hydrogen peroxide is associated with altered production of inflammatory mediators and antioxidants by alveolar macrophages

Hydrogen peroxide (H2O2) is present in the atmosphere at concentrations known to induce cell and tissue damage. However, inhaled H2O2 vapor should not reach the lower lung due to its high water solubility. It has been suggested that hygroscopic components of particulate matter (PM) may transport H2O2 into the lower lung and induce tissue injury and this was investigated. Ammonium sulfate [(NH4)(2)SO4] was selected as a model for fine atmospheric PM. Treatment of female Sprague-Dawley rats with (NH4)(2)SO4 (429 or 215 mug/m(3); 0.3-0.4 mum mass median diameter) or H2O2 (10, 20, or 100 ppb) alone or in combination for 2 h had no major effect on bronchoalveolar lavage fluid cell number or viability or on protein content or lactate dehydrogenase levels, either immediately or 24 h after exposure, relative to air-exposed rats. However, electron microscopy revealed increased numbers of neutrophils in pulmonary capillaries adhered to the vascular endothelium in rats treated with the combination of (NH4)(2)SO4 + H2O2. Exposure of rats to (NH4)(2)SO4 + H2O2 also resulted in tumor necrosis factor-alpha (TNF-alpha) production by alveolar macrophages. This was observed immediately and 24 h after exposure. Immediately after inhalation of (NH4)(2)SO4 + H2O2, a transient increase in production of superoxide anion by alveolar macrophages was observed. In contrast, nitric oxide production by cells from rats exposed to (NH4)(2)SO4 + H2O2 or H2O2 alone was decreased, and this persisted for 24 h. Decreases in nitric oxide may be due to superoxide anion-driven formation of peroxynitrite. In this regard, nitrotyrosine, an in vivo marker of peroxynitrite, was detected in lung tissue after exposure of rats to (NH4)(2)SO4 + H2O2 or H2O2. We also found that expression of the antioxidant enzyme heme oxygenase-1 by stimulated alveolar macrophages was increased following exposure of rats to (NH4)(2)SO4 + H2O2. Taken together, these studies demonstrate that the biological effects of inhaled fine PM are augmented by H2O2. Moreover, tissue injury induced by fine PM may be related to altered production of cytotoxic mediators by alveolar macrophages. (C) 2001 Elsevier Science.