Nitidine Chloride inhibits cell proliferation and invasion via downregulation of YAP expression in prostate cancer cells

被引:2
作者
Shi, Ying [1 ]
Cao, Tong [2 ]
Sun, Yu [1 ]
Xia, Jun [1 ]
Wang, Peter [1 ]
Ma, Jia [1 ]
机构
[1] Bengbu Med Coll, Sch Lab Med, Dept Biochem & Mol Biol, Bengbu 233030, Anhui, Peoples R China
[2] Bengbu Med Coll, Dept Clin Lab, Affiliated Hosp 1, Bengbu 233004, Anhui, Peoples R China
来源
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH | 2019年 / 11卷 / 02期
关键词
YAP; prostate cancer; nitidine chloride; hippo; growth; TO-MESENCHYMAL TRANSITION; HIPPO PATHWAY; SIGNALING PATHWAY; CARCINOMA-CELLS; UP-REGULATION; APOPTOSIS; MIGRATION; GROWTH; DOXORUBICIN; METASTASIS;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Nitidine chloride (NC) exhibits tumor suppressive function in a variety of human cancers. However, the molecular mechanism of NC-triggered anti-cancer activity has not been fully elucidated. In the present study, we aim to investigate the anti-tumor molecular basis of NC in prostate cancer cells. Multiple approaches including MTT, FACS, wound healing assay, Transwell invasion assay, Transfection, and Western blotting were performed. We found that NC inhibited cell growth and induced apoptosis in prostate cancer cells. Moreover, NC suppressed cell migration and invasion in prostate cancer cells. Notably, we found that NC decreased the expression of YAP oncoprotein in prostate cancer cells. Downregulation of YAP enhanced the anti-tumor function mediated by NC in prostate cancer cells. On the contrary, upregulation of YAP abrogated the anti-cancer activity of NC treatment in prostate cancer cells. Our findings indicate that NC could be useful as a YAP inhibitor for the treatment of prostate cancer cells.
引用
收藏
页码:709 / 720
页数:12
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