Physalis angulata extract exerts anti-inflammatory effects in rats by inhibiting different pathways

被引:64
作者
Bastos, G. N. T. [1 ]
Silveira, A. J. A. [2 ,3 ]
Salgado, C. G. [4 ,5 ]
Picanco-Diniz, D. L. W. [6 ]
do Nascimento, J. L. M. [1 ]
机构
[1] Fed Univ Para, Inst Ciencias Biol, Lab Neuroquim Mol & Cellular, BR-66075900 Belem, Para, Brazil
[2] Fed Univ Para, Inst Ciencias Exatas & Nat, Dept Quim, BR-66075900 Belem, Para, Brazil
[3] Fed Univ Para, Nucleo Med Trop, BR-66055240 Belem, Para, Brazil
[4] Fed Univ Para, Inst Ciencias Biol, Dept Patol, BR-66075900 Belem, Para, Brazil
[5] Unidade Referencia Dr Marcello Candia, BR-67200000 Marituba, Para, Brazil
[6] Fed Univ Para, Inst Ciencias Biol, Dept Fisiol, Neuroendocrinol Lab, BR-66075900 Belem, Para, Brazil
关键词
Physalis angulata L; prostaglandin E; adenosine deaminase; nitric oxide; TGF-beta; anti-inflammatory response; Amazon ethnopharmacology;
D O I
10.1016/j.jep.2008.04.005
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Physalis angulata is a popular medicine used in Brazil due to its anti-inflammatory effects, but the pharmacological mechanisms underlying these actions remain to be better understood. In the present work, lyorphilized aqueous extract from the roots of Physalis angulata Linneu (AEPa) was used to control the inflammatory response induced by the injection of 1% carrageenan into subcutaneous rat's air pouches. Adenosine deaminase (ADA) activity, nitrite level, and prostaglandin E-2 (PGE(2)) level were used to evaluate the action of inflammatory mediators. Tumor growth factor-beta (TGF-beta) level was used as a bioindicator of immunomodulatory response. Rats were injected with vehicle, indomethacin, or AEPa (0.5 mg/kg, 1 mg/kg, and 5 mg/kg i.p.), 1 h before carrageenan administration. AEPa at 0.5 mg/kg had no effect. However, 1 mg/kg of AEPa showed significant anti-inflammatory effects, decreasing exudate volume, total number of inflammatory cells, ADA activity, nitrite level, and PGE2 level in 50%, 41%, 20%, 60%, and 41%, respectively. The anti-inflammatory effects of 5 mg/kg AEPa appeared to be more effective than those of 1 mg/kg AEPa (84%, 80%, 43%, 70%, and 75%, respectively). In addition, TGF-beta level was upregulated to 9700 pg/ml after 5 mg/kg AEPa, in comparison with 160 pg/ml in the vehicle-treated group, and 137 pg/ml in the indomethacin-treated group. The results indicate that AEPa exerts powerful anti-inflammatory and immunomodulatory activities, interfering with the cyclooxygenase pathway, lymphocyte proliferation, NO, and TGF-beta production. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:246 / 251
页数:6
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