Isolation and characterization of 19 polymorphic microsatellite DNA markers in the Japanese brown frog (Rana japonica)

被引：3

作者：

Koizumi, Noriyuki ^{[1
]}

Watabe, Keiji ^{[1
]}

Mori, Atsushi ^{[1
]}

Takemura, Takeshi ^{[1
]}

机构：

[1] Natl Inst Rural Engn, Tsukuba, Ibaraki 3058609, Japan

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 01期

基金：

日本学术振兴会;

关键词：

conservation; Japanese brown frog; microsatellite; paddy field; population genetics; SOFTWARE;

D O I：

10.1111/j.1755-0998.2008.02429.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the isolation and characterization of 19 polymorphic microsatellite DNA markers in the Japanese brown frog (Rana japonica). These markers were tested in 24 individuals each collected from three distinct populations in Ichikai-machi, Tochigi Prefecture. The number of observed alleles per locus ranged from 3 to 24 across all populations, and the values of observed and expected heterozygosities ranged from 0.130 to 1 and from 0.125 to 0.941, respectively. These microsatellite loci will be useful for investigating the intraspecific genetic variation and population structure of this species.

引用

页码：248 / 250

页数：3

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