Characterization of eight polymorphic loci for Maasai giraffe (Giraffa camelopardalis tippelskirchi) using non-invasive genetic samples

被引：2

作者：

Crowhurst, Rachel S. ^{[1
]}

Mullins, Tom D. ^{[2
]}

Mutayoba, Benezeth M. ^{[3
]}

Epps, Clinton W. ^{[1
]}

机构：

[1] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA

[2] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis, OR 97331 USA

[3] Sokoine Univ Agr, Dept Vet Physiol Biochem Pharmacol & Toxicol, Morogoro, Tanzania

来源：

CONSERVATION GENETICS RESOURCES | 2013年 / 5卷 / 01期

基金：

美国国家科学基金会;

关键词：

Giraffa camelopardalis; Microsatellite; Illumina sequencing; Non-invasive sampling;

D O I：

10.1007/s12686-012-9739-x

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We used Illumina sequencing to develop eight novel microsatellite loci for Maasai giraffe (Giraffa camelopardalis tippelskirchi), and screened them using fecal DNA. Genetic diversity was assessed for 40 individuals from the Katavi National Park/Rukwa Game Reserve ecosystem in Tanzania. The number of alleles per locus ranged from 2 to 14 (mean = 6.2) and mean expected heterozygosity was moderate (range 0.025-0.838, mean = 0.527). These markers were successfully tested using degraded DNA and may be useful for future studies of giraffe, especially those that use non-invasive sampling techniques.

引用

页码：85 / 87

页数：3

共 13 条

[1]

[Anonymous], 2011, The IUCN Red List of Threatened Species

[2] Extensive population genetic structure in the giraffe [J].

Brown, David M. ;

Brenneman, Rick A. ;

Koepfli, Klaus-Peter ;

Pollinger, John P. ;

Mila, Borja ;

Georgiadis, Nicholas J. ;

Louis, Edward E., Jr. ;

Grether, Gregory F. ;

Jacobs, David K. ;

Wayne, Robert K. .

BMC BIOLOGY, 2007, 5 (1)

[3] Development of 11 microsatellite markers for Giraffa camelopardalis through 454 pyrosequencing, with primer options for an additional 458 microsatellites [J].

Carter, Kerryn D. ;

Seddon, Jennifer M. ;

Carter, John K. ;

Goldizen, Anne W. ;

Hereward, James P. .

CONSERVATION GENETICS RESOURCES, 2012, 4 (04) :943-945

[4]

Craig DW, 2008, NAT METHODS, V5, P887, DOI [10.1038/nmeth.1251, 10.1038/NMETH.1251]

[5] Multiplex sequencing of plant chloroplast genomes using Solexa sequencing-by-synthesis technology [J].

Cronn, Richard ;

Liston, Aaron ;

Parks, Matthew ;

Gernandt, David S. ;

Shen, Rongkun ;

Mockler, Todd .

NUCLEIC ACIDS RESEARCH, 2008, 36 (19)

[6]

Crowhurst R, 2012, THESIS OREGON STATE

[7]

Dagg AI., 1976, GIRAFFE ITS BIOL BEH

[8]

Fennessy J, 2010, IUCN 2011 IUCN RED L

[9] Characterization of 16 microsatellite marker loci in the Maasai giraffe (Giraffa camelopardalis tippelskirchi) [J].

Huebinger, RM ;

Pierson, DJ ;

De Maar, TW ;

Brown, DM ;

Brenneman, RA ;

Louis, EE .

MOLECULAR ECOLOGY NOTES, 2002, 2 (04) :531-533

[10]

Kingdon J., 1997, KINGDON FIELD GUIDE

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