Development of Poliovirus Extraction Method from Stool Extracts by Using Magnetic Nanoparticles Sensitized with Soluble Poliovirus Receptor

被引:8
作者
Arita, Minetaro [1 ]
机构
[1] Natl Inst Infect Dis, Dept Virol 2, Tokyo, Japan
基金
比尔及梅琳达.盖茨基金会;
关键词
DEOXYINOSINE RESIDUES; CODON DEGENERACY; MIXED-BASE; IDENTIFICATION; PRIMERS; POSITIONS; SEROTYPE;
D O I
10.1128/JCM.00499-13
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude biological samples.
引用
收藏
页码:2717 / 2720
页数:4
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