A Solid-State Electrochemiluminescence Ethanol Biosensor Based on Electrogenerated Poly(pyrrole-tris(2,2-bipyridyl)ruthenium(II)) Film/Alcohol Dehydrogenase/Laponite Composite

被引：7
作者：
Gao, Bu-Hong [1 ]
Ding, Shou-Nian [1 ]
Wang, Yi-Hong [1 ]
Dai, Yunqian [1 ]
Xia, Jiangyan [2 ]
Sun, Yue-Ming [1 ]
Cosnierc, Serge [3 ]
机构：
[1] Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Jiangsu, Peoples R China
[2] Southeast Univ, Southeast Univ Affiliated Zhongda Hosp, Nanjing 210009, Peoples R China
[3] Univ Grenoble 1, Dept Chim Mol UMR 5250, ICMG FR 2607, CNRS, F-38041 Grenoble, France
来源：
ELECTROANALYSIS | 2013年 / 25卷 / 03期
关键词：
Electrochemiluminescence; Biosensors; Laponite; Alcohol dehydrogenase; GLASSY-CARBON ELECTRODE; CHEMILUMINESCENCE; TRIS(2,2'-BIPYRIDYL)RUTHENIUM(II); NANOPARTICLES; RU(BPY)(3)(2+); FILMS; SURFACE;
D O I：
10.1002/elan.201200626
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Based on the solid-state electrochemiluminescence (ECL) of an electrogenerated polypyrrole tagged with tris(2,2-bipyridyl)ruthenium(II) complex ( denoted as polypyr-Ru), a new ethanol biosensor was fabricated by casting the composite of alcohol dehydrogenase (ADH) and laponite colloids onto the polypyr-Ru /Pt electrode. The two layer structure keeps the good robustness of the biosensor due to the electrostatic interaction between the negatively charged biocomposite coating and the positively charged polypyr-Ru. The morphology of the laponite-ADH composite coating is characterized by scanning electron microscopy. The electrochemical and ECL properties of the modified electrodes are investigated by cyclic voltammetry. The parameters concerning the ECL biosensor such as the amount of enzyme usage, the appropriate pH are optimized. The resulted ECL biosensor shows a good performance to detect ethanol.
引用
收藏
页码：697 / 702
页数：6
相关论文
共 32 条
[1]   Improvement of poly(amphiphilic pyrrole) enzyme electrodes via the incorporation of synthetic laponite-clay-nanoparticles [J].
Besombes, JL ;
Cosnier, S ;
Labbe, P .
TALANTA, 1997, 44 (12) :2209-2215
[2]   Recent advances in electrochemiluminescent enzyme biosensors [J].
Chen, Xiao-mei ;
Su, Bing-yuan ;
Song, Xin-hong ;
Chen, Qing-ai ;
Chen, Xi ;
Wang, Xiao-ru .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2011, 30 (05) :665-676
[3]   Electrogenerated chemiluminescence from tris(2,2′-bipyridyl)ruthenium(II) immobilized in titania-perfluorosulfonated lonomer composite films [J].
Choi, HN ;
Cho, SH ;
Lee, WY .
ANALYTICAL CHEMISTRY, 2003, 75 (16) :4250-4256
[4]   Electrochemiluminescence of ruthenium(II) tris(bipyridine) encapsulated in sol-gel classes [J].
Collinson, MM ;
Novak, B ;
Martin, SA ;
Taussig, JS .
ANALYTICAL CHEMISTRY, 2000, 72 (13) :2914-2918
[5]   Tris(2,2′-bipyridyl)ruthenium(II)-zirconia-nafion composite modified electrode applied as solid-state electrochemiluminescence detector on electrophoretic microchip for detection of pharmaceuticals of tramadol, lidocaine and ofloxacin [J].
Ding, Shou-Nian ;
Xu, Jing-Juan ;
Zhang, Wen-Jia ;
Chen, Hong-Yuan .
TALANTA, 2006, 70 (03) :572-577
[6]   Enhanced solid-state electrochemiluminescence of Ru(bpy)32+ immobilized on a laponite gel-state network and its glucose biosensing application [J].
Ding, Shou-Nian ;
Gao, Bu-Hong ;
Shan, Dan ;
Cosnier, Serge ;
Sun, Yue-Ming .
RSC ADVANCES, 2012, 2 (29) :10813-10816
[7]   TiO2 nanocrystals electrochemiluminescence quenching by biological enlarged nanogold particles and its application for biosensing [J].
Ding, Shou-Nian ;
Gao, Bu-Hong ;
Shan, Dan ;
Sun, Yue-Ming ;
Cosnier, Serge .
BIOSENSORS & BIOELECTRONICS, 2013, 39 (01) :342-345
[8]   Single-Walled Carbon Nanotubes Noncovalently Functionalized by Ruthenium(II) Complex Tagged with Pyrene: Electrochemical and Electrogenerated Chemiluminescence Properties [J].
Ding, Shou-Nian ;
Shan, Dan ;
Cosnier, Serge ;
Le Goff, Alan .
CHEMISTRY-A EUROPEAN JOURNAL, 2012, 18 (37) :11564-11568
[9]   Dramatically Enhanced Solid-State Electrochemiluminescence of CdTe Quantum Dots Composed with TiO2 Nanoparticles [J].
Ding, Shou-Nian ;
Gao, Bu-Hong ;
Shan, Dan ;
Sun, Yue-Ming ;
Cosnier, Serge .
CHEMISTRY-A EUROPEAN JOURNAL, 2012, 18 (06) :1595-1598
[10]   Electrogenerated chemiluminescence of poly[(2,2'-bipyridyl) (4-(2-pyrrol-1-ylethyl)-4'-methyl-2,2'-bipyridyl)2]ruthenium (II) film [J].
Ding, Shou-Nian ;
Cosnier, Serge ;
Shan, Dan ;
Sun, Yue-Ming ;
Wang, Yao .
ELECTROCHEMISTRY COMMUNICATIONS, 2010, 12 (07) :905-908
1234