Bioinformatics Interaction Analysis of Wnt1-Targeting miRNAs and Their Corresponding circRNAs and Target Genes

To predict circRNAs and target genes for seven known Wnt1-targeting miRNAs, then further analyze the interaction among these seven miRNAs and their corresponding circRNAs and target genes, seven reported Wnt1-targeting miRNAs including let-7e, miR-21, miR-34a, miR-122, miR-148a, miR-148b and miR-152 were predicted for their interacting circRNAs and target genes by Starbase and miRWALK softwares, respectively. Then the network analysis of miRNA and their interaction circRNAs and target genes was built by Cytoscape3.2.1 software. DAVID was performed to analyze singaling pathway according to miRNAs predicted target genes. The number of target circRNAs predicted by Starbase software for these 7 different miRNAs were 58, 15, 41, 20, 28, 28 and 28, respectively. Comparing the relationship between miRNAs and their target gene in more than 7 9 softwares predicted by miRWALK, we found that the CHD7 gene is the only one corresponding with miR-21, miR-148-a and miR-148 and miR-152 in more than 7 softwares predications. CNOT6, NBEA, ZFYVE26 and ZDHHC17 were the target genes equivalent with at least 4 miRNAs predicted by both 7 and 8 different softwares in miRWALK. For KEGG signaling pathways, Fool adhesion, MAPK, Notch and TGF-beta signaling pathways were all predicted by seven miRNAs' target genes in more than 7 different predication softwares of miRWALK. In the MAPK signaling pathway, DUSP1 was found to have interaction with miR-21,miR-148a, miR-148b and miR-152 simultaneously, MRPS35_hsa_circ_001042 was also predicted to interact with these four miRNAs. We found that the DUSP1 and MRPS35_hsa_circ_001042 in the MAPK signaling pathway, are the target genes and circRNA, interacted with miR-21,miR-148a, miR-148b, and miR-152, respectively. In the study, we analyzed known Wnt1-targeting miRNAs and their corresponding circRNAs and predicted target genes, signaling pathway and interaction network were also involved. The study laid the foundation for the further analysis of the interaction among Wnt1-targeting miRNAs, circRNAs and target genes.