Differences in neuropeptide Y-induced secretion of endothelin-1 in left and right human endocardial endothelial cells

被引：17

作者：

Abdel-Samad, Dima ^{[1
]}

Perreault, Claudine ^{[1
]}

Ahmarani, Lena ^{[1
]}

Avedanian, Levon ^{[1
]}

Bkaily, Ghassan ^{[1
]}

Magder, Sheldon ^{[2
]}

D'Orleans-Juste, Pedro ^{[3
]}

Jacques, Danielle ^{[1
]}

机构：

[1] Univ Sherbrooke, Fac Med, Dept Anat & Cell Biol, Sherbrooke, PQ J1H 5N4, Canada

[2] McGill Univ, Ctr Hlth, Royal Victoria Hosp, Montreal, PQ H3A 1A1, Canada

[3] Univ Sherbrooke, Fac Med, Dept Pharmacol, Sherbrooke, PQ J1H 5N4, Canada

来源：

NEUROPEPTIDES | 2012年 / 46卷 / 06期

基金：

加拿大健康研究院;

关键词：

Neuropeptide Y; NPY receptors; Endocardial endothelial cells; Endothelin-1; ET-1; secretion; GPCR; Human endothelium; Excitation-secretion coupling; NPY receptor antagonist; Y-5; receptor; VASCULAR ENDOTHELIUM; RECEPTOR; CARDIOMYOCYTES; BIIE0246; HEART;

D O I：

10.1016/j.npep.2012.09.003

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The aim of the study was to test the hypothesis that neuropeptide Y (NPY) may induce endothelin-1 (ET-1) secretion in left (hLEECs) and right (hREECs) human endocardial endothelial cells. Furthermore, the type of NPY receptor implicated could be different in NPY-induced secretion in hLEECs and hREECs. Using immunofluorescence coupled to real 3D confocal microscopy and ELISA, our results showed that stimulation of secretion by NPY induced the release of ET-1 from both right and left human ventricular endocardial endothelial cells (hEECs) in a time-dependent manner. Furthermore, the secretory capacity of hREECs was higher than that of hLEECs. In addition, our results showed that the effect of NPY on ET-1 secretion in hLEECs was only due to activation of Y-5 receptors. However, the effect of NPY on ET-1 secretion in hREECs was due to mainly Y-2 and partially Y-5 receptors activation. In conclusion, our results suggest that differences in excitation-secretion coupling exist between hREECS and hLEECs which may contribute to the functional differences between right and left ventricular muscle. Furthermore, high NPY level contributes to ET-1 release by hEECs and Y-2 and Y-5 receptors antagonists may be used for regulation of ET-1 secretion in the heart. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：373 / 382

页数：10

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