Analysis of polycrystallinity in hen egg-white lysozyme using a pnCCD

被引:21
作者
Send, Sebastian [1 ]
Abboud, Ali [1 ]
Leitenberger, Wolfram [2 ]
Weiss, Manfred S. [3 ]
Hartmann, Robert [4 ]
Strueder, Lothar [1 ,5 ,6 ]
Pietsch, Ullrich [1 ]
机构
[1] Univ Siegen, Dept Phys, D-57068 Siegen, Germany
[2] Univ Potsdam, Dept Phys, Potsdam, Germany
[3] Helmholtz Zentrum Berlin Mat & Energie GmbH, MX Grp, Berlin, Germany
[4] PNSensor GmbH, Munich, Germany
[5] Max Planck Inst Halbleiterlabor, Munich, Germany
[6] Max Planck Inst Extraterr Phys, D-37075 Garching, Germany
关键词
DIFFRACTION;
D O I
10.1107/S0021889812015038
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A crystal of hen egg-white lysozyme was analyzed by means of energy-dispersive X-ray Laue diffraction with white synchrotron radiation at 2.7 angstrom resolution using a pnCCD detector. From Laue spots measured in a single exposure of the arbitrarily oriented crystal, the lattice constants of the tetragonal unit cell could be extracted with an accuracy of about 2.5%. Scanning across the sample surface, Laue images with split reflections were recorded at various positions. The corresponding diffraction patterns were generated by two crystalline domains with a tilt of about 1 degrees relative to each other. The obtained results demonstrate the potential of the pnCCD for fast X-ray screening of crystals of macromolecules or proteins prior to conventional X-ray structure analysis. The described experiment can be automatized to quantitatively characterize imperfect single crystals or polycrystals.
引用
收藏
页码:517 / 522
页数:6
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