Assessment on pollution of ochratoxin a in grain in China and its apoptosis effect on vitro-cultured human tubular kidney cells

被引：18

作者：

Li, Zengning ^{[1
]}

Zhang, Xianghong ^{[2
]}

Cui, Jinfeng ^{[2
]}

Kang, Weijun ^{[3
]}

机构：

[1] Hebei Med Univ, Attached Hosp 1, Dept Nutr, Shijiazhuang, Peoples R China

[2] Hebei Med Univ, Dept Pathol, Shijiazhuang, Peoples R China

[3] Hebei Med Univ, Sch Publ Hlth, Shijiazhuang, Peoples R China

来源：

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2012年 / 26卷 / 04期

关键词：

Ochratoxin A; Apoptosis; Signaling Pathway; C-Jun NH2 Terminal Kinase; Mitogen-Activated Protein Kinase; ENDEMIC NEPHROPATHY; SIGNAL-TRANSDUCTION; IN-VITRO; EXPOSURE; RATS; NEPHROTOXICITY; ACTIVATION; MECHANISMS; PATHWAY; JNK;

D O I：

10.1002/jbt.20420

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ochratoxin A (OTA) is nephrotoxic, immunosuppressive, and teratogenic in many species and is a possible human carcinogen. In this study, we investigated the OTA pollution situations of grains in northern China and the signaling pathway that mediated OTA-induced apoptosis in human tubular kidney cells (HKCs). Samples of grains collected from three representative areas were determined by using high-performance liquid chromatography fluorescence method. The effects of OTA on cell apoptosis, caspase-3, Bax, and Bcl-2 expression, and phosphorylation of c-Jun NH2 terminal kinase (JNK) were detected in cultured HKCs via flow cytometry (FCM), Hoechst 33258 staining, and Western blot. It showed that OTA pollution of edible grains was very common in north China. OTA could affect caspase-3, Bax, and Bcl-2 expression and increased cell apoptosis in cultured HKCs. The JNK signalling pathway might play an important role during these cellular events. (C) 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:139146, 2012; View this article online at . DOI 10.1002/jbt.20420

引用

页码：139 / 146

页数：8

共 34 条

[1] OCHRATOXIN-A PRODUCTION BY STRAINS OF ASPERGILLUS-NIGER VAR NIGER [J].

ABARCA, ML ;

BRAGULAT, MR ;

CASTELLA, G ;

CABANES, FJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (07) :2650-2652

[2] Distribution of ochratoxin A in plasma and tissues of rats fed a naturally contaminated diet amended with micronized wheat fibres: Effectiveness of mycotoxin sequestering activity [J].

Aoudia, N. ;

Tangni, E. K. ;

Larondelle, Y. .

FOOD AND CHEMICAL TOXICOLOGY, 2008, 46 (03) :871-878

[3] Ochratoxin A induces apoptosis in human lymphocytes through down regulation of Bcl-xL [J].

Assaf, H ;

Azouri, H ;

Pallardy, M .

TOXICOLOGICAL SCIENCES, 2004, 79 (02) :335-344

[4] Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms [J].

Bonni, A ;

Brunet, A ;

West, AE ;

Datta, SR ;

Takasu, MA ;

Greenberg, ME .

SCIENCE, 1999, 286 (5443) :1358-1362

[5] Assessment of the exposure to ochratoxin A in the province of Lleida, Spain [J].

Coronel, M. B. ;

Sanchis, V. ;

Ramos, A. J. ;

Marin, S. .

FOOD AND CHEMICAL TOXICOLOGY, 2009, 47 (11) :2847-2852

[6] Signal transduction by the JNK group of MAP kinases [J].

Davis, RJ .

CELL, 2000, 103 (02) :239-252

[7] Influencing factors on bread-derived exposure to ochratoxin A: Type, origin and composition [J].

Duarte, S. C. ;

Bento, J. ;

Pena, A. ;

Lino, C. M. ;

Delerue-Matos, C. ;

Oliveira, M. B. P. P. ;

Alves, M. R. ;

Pereira, J. A. .

FOOD AND CHEMICAL TOXICOLOGY, 2010, 48 (8-9) :2139-2147

[8] Ochratoxin A-induced renal cortex fibrosis and epithelial-to-mesenchymal transition: Molecular mechanisms of ochratoxin A-injury and potential effects of red wine [J].

Gagliano, N ;

Torri, C ;

Donetti, E ;

Grizzi, F ;

Costa, F ;

Bertelli, AA ;

Migliori, M ;

Filippl, C ;

Bedoni, M ;

Panichi, V ;

Giovannini, L ;

Gioia, M .

MOLECULAR MEDICINE, 2005, 11 (1-12) :30-38

[9] Early cytotoxic effects of ochratoxin A in rat liver: A morphological, biochemical and molecular study [J].

Gagliano, Nicoletta ;

Donne, Isabella Dalle ;

Torri, Carlo ;

Migliori, Massimiliano ;

Grizzi, Fabio ;

Milzani, Aldo ;

Filippi, Cristina ;

Annoni, Giorgio ;

Colombo, Piergiuseppe ;

Costa, Francesco ;

Ceva-Grimaldi, Giorgia ;

Bertelli, Alberto A. E. ;

Giovannini, Luca ;

Gioia, Magda .

TOXICOLOGY, 2006, 225 (2-3) :214-224

[10] FORMATION OF OCHRATOXIN-A METABOLITES AND DNA-ADDUCTS IN MONKEY KIDNEY-CELLS [J].

GROSSE, Y ;

BAUDRIMONT, I ;

CASTEGNARO, M ;

BETBEDER, AM ;

CREPPY, EE ;

DIRHEIMER, G ;

PFOHLLESZKOWICZ, A .

CHEMICO-BIOLOGICAL INTERACTIONS, 1995, 95 (1-2) :175-187

← 1 2 3 4 →